The objective of this study was to analyze the genetic polymorphism of PRKACB gene se-quence in Angora rabbits and its correlation with production traits(body weight,wool yield),and to iden-tify molecular markers in relation to wool production,and to provide reference for accelerating the breed-ing of Angora rabbits.Direct sequencing was used to detect SNPs in the PRKACB gene exon and promoter region of 169 Angora rabbits,and SPSS 25.0 software was used to analyze the correlation between SNPs and weight and wool production.Results showed PRKACB gene has 10 exons and 9 introns,with the exon sequences being relatively conserved and no SNPs detected.In the 1366bp promoter sequence,two SNP lo-ci(rs81065607 A>C and rs81066149 C>T)were found,both containing three genotypes and conforming to Hard-Weinberg equilibrium.The polymorphic information content values of rs81065607 A>C and rs81066149 C>T were 0.331 and 0.327,indicating moderate polymorphism,suggesting that the PRKACB gene has high genetic variability in the Angora rabbit population.The linkage disequilibrium analysis showed a high linkage between the two SNP loci(r2=0.983).The association analysis showed that the in-dividuals with the CC genotypes of rs81065607 A>C had significantly higher wool production than those with AA and AC genotypes(P<0.05);individuals with the TT genotype of rs81066149 C>T had signifi-cantly higher wool yield than those with CC and CT genotypes(P<0.05).It is therefore concluded that the SNP sites rs81065607 A>C and rs81066149 C>T in the PRKACB gene can be potential molecular markers for wool yield in Angora rabbits,and can be used in molecular breeding of Angora rabbits.
维普
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