Interaction of miRNA-134 with MMP-9 in patients with severe middle cerebral artery stenosis ischemic stroke and assessment of collateral circulation formation
Objective To explore the interaction between microRNA-134(miRNA-134)and Matrix Metalloproteinase-9(MMP-9)in patients with severe middle cerebral artery stenosis ischemic stroke and to assess the formation of collateral circulation.Methods A prospective study was conducted from August 2021 to August 2022,including 90 patients with severe middle cerebral artery stenosis ischemic stroke treated in the Department of Neurology at Xingtai Third Hospital,with 45 patients having poor collateral circulation and 45 having good collateral circulation;additionally,90 healthy individuals from the same period served as a control group.Patient demographic data were collected,and serum levels were measured.The diagnostic value of serum miRNA-134 and MMP-9 levels in patients with severe middle cerebral artery stenosis ischemic stroke was analyzed using the Receiver Operating Characteristic(ROC)curve.The correlation between miRNA-134 and MMP-9 in serum was analyzed using Pearson correlation,and a multivariate logistic regression model was used to analyze the factors affecting poor collateral circulation in these patients.Results The observation group had significantly higher levels of triglycerides and systolic blood pressure compared to the control group(t=12.743,21.940,P<0.05).Serum levels of miRNA-134,homocysteine(Hcy),high-sensitivity C-reactive protein(hs-CRP),and MMP-9 in the observation group were significantly higher than those in the control group(t=14.862,23.229,21.649,20.868,P<0.05).ROC curve analysis indicated that both miRNA-134 and MMP-9 have certain diagnostic value in patients with severe ischemic stroke,with AUCs of 0.875 and 0.812,respectively,and their combined application showed even higher diagnostic value,with an AUC of 0.942.Pearson correlation analysis revealed a positive correlation between the expression levels of miRNA-134 and MMP-9 in the serum of patients with severe middle cerebral artery stenosis ischemic stroke(r=0.783,P<0.05).Multivariate logistic regression analysis identified systolic blood pressure,triglycerides,MMP-9,and miRNA-134 as factors influencing the poor collateral circulation in patients with severe middle cerebral artery stenosis ischemic stroke(P<0.05).Conclusion Serum levels of miRNA-134 and MMP-9 are significantly elevated in patients with severe middle cerebral artery stenosis ischemic stroke,and there is a correlation between them,which can provide a reference for the diagnosis of poor collateral circulation in these patients.
万方数据
维普
