首页|缺氧对大鼠6种药物转运体基因表达影响的差异性研究

缺氧对大鼠6种药物转运体基因表达影响的差异性研究

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目的 初步探讨缺氧时Pept1、Oatp1b1、Oat1、Oct1、Mdr1和Mrp2 6种药物转运体基因表达的变化.方法 Wistar大鼠随机分为正常对照组、缺氧24 h和72 h组,缺氧组置于模拟海拔5000 m(压力:397.5 mmHg,氧分压:42 mmHg)的低压氧舱中.提取各组大鼠小肠、肝脏、肾脏组织中的总RNA,以Real-time PCR方法检测6种药物转运体基因表达量.结果 与正常对照组相比,缺氧组中6种药物转运体基因表达大多数升高,但随着时间的延长,不同组织中的不同转运体出现不一样的变化趋势.结论 高原缺氧会影响药物转运体mRNA的表达水平,这可为高原药物转运体及药代动力学研究提供一定的理论依据.
Difference in Effects of Hypoxia on Gene Expressions of Six Drug Transporters in Rats
Objective To study the gene expressions of six drug transporters-Pept1,Oatp1b1,Oat1,Oct1,Mdr1 and Mrp2 in rats after acute hypoxia at high altitude.Methods Wistar rats were randomly divided into normal control group,hypoxia 24 h group and hypoxia 72 h group.The experimental groups (hypoxia 24 h and 72 h groups) were fed in a hypobaric chamber simulated to an altitude of 5000 m (pressure:397.5 mmHg,the oxygen partial pressure:42 mmHg).Total RNA was extracted from the small intestine,liver and kidney of rats in each group.Real-time PCR was used to detect the expression levels of six transporter genes.Results Compared with control group,the expressions of most of the drug transporters Pept1,Oatp1b1,Oat1,Oct1,Mdr1 and Mrp2 in hypoxia groups were increased,but different transporters in different tissues developed diverse trends with the extension of hypoxia time.Conclusion Hypoxia at high altitude can affect the mRNA expression levels of drug transporters,which may provide some reference for the study of drug transporters and pharmacokinetics at high altitude.

hypoxiadrug transportersPCRgene expressionrats

靳婷、罗冰峰、张晓云、李文斌、张娟红、张明霞、王昌、赵安鹏、王荣

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730000甘肃兰州,兰州大学药学院

730050甘肃兰州,兰州军区兰州总医院全军高原损伤防治重点实验室

缺氧 药物转运体 PCR 基因表达 大鼠

国家科技重大专项国家自然科学基金国家自然科学基金国家自然科学基金全军后勤科研“十二五”重大项目全军医学科研“十二五”重点项目甘肃省自然科学基金甘肃省自然科学基金

2008ZXJ09014-010814015528140300481673508AWS14L0005BWS12J012145RJZA1111606RJZA176

2017

10.3969/j.issn.1008-9926.2017.04.001

解放军药学学报
中国人民解放军总后勤部卫生部 药品仪器检验所

解放军药学学报

CSTPCDCSCD
影响因子：0.529
ISSN：1008-9926
年,卷(期)：2017.33(4)
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