复方三七滴丸质量标准研究
Determination of Compound Sanqi Dripping Pills by HPLC
李思治 1王剑丽 1刘彬果1
作者信息
- 1. 300142 天津,联勤保障部队第983医院
- 折叠
摘要
目的 建立复方三七滴丸的质量标准.方法 采用薄层色谱法对制剂中的三七进行定性鉴别,高效液相色谱法测定丹参中丹参素的含量,采用ZORBAX SB-C18 色谱柱(4.6 mm×250 mm,5 μm);流动相为甲醇-水-冰醋酸(8∶91∶1);流速:1.0 ml·min-1;检测波长:280 nm.结果 薄层色谱鉴别样品斑点清晰,阴性对照无干扰;在0.5～10.0 μg的范围内,丹参素钠呈良好的线性关系(r=0.9992,n=7).加样回收率为99.8%,RSD值为0.6%.结论 建立的质量标准方法准确、重复性好、结果可靠,能有效控制复方三七滴丸的质量.
Abstract
Objective To establish a quality control method for compound Sanqi dripping pills.Methods Notoginseng in compound Sanqi dripping pills was identified via TLC.The content of salvianic acid A sodium was determined by HPLC with the ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm).The mobile phrase was composed of methanl-water-1%acetic acid(8∶91∶1)at a flow rate of 1.0 ml·min-1 and detected at 280 nm.Results The spots in TLC were clear without any interference.Salvianic acid A showed a good linearity in the range of 0.5 to 10.0 μg(r=0.9992,n=7).The average recovery was 99.8%,with an RSD of 0.6%.Conclusion This method is simple and reliable,which can be used for qual-ity control of compound Sanqi pills.
关键词
高效液相色谱法/复方三七滴丸/丹参素Key words
HPLC/compound Sanqi dripping pills/salvianic acid A引用本文复制引用
出版年
2024
NETL
NSTL
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