水芹总酚酸体外抗HIV-1的作用
In Vitro Anti HIV-1 Effect of Total Phenolics Acid Extracted from Oenanthe Javanica
黄紫晴 1于珊 2黄正明 3马丽英 4刘青川3
作者信息
- 1. 100039 北京,北京卫健基业生物技术研究所;110016 辽宁沈阳,沈阳药科大学无涯创新学院
- 2. 113000 辽宁抚顺,抚顺市检验检测认证中心
- 3. 100039 北京,北京卫健基业生物技术研究所
- 4. 100050 北京,中国疾病预防控制中心性病艾滋病预防控制中心
- 折叠
摘要
目的 探讨水芹总酚酸体外抑制人类免疫缺陷病毒1型的作用.方法 用不同稀释度的水芹总酚酸与TZM-bl细胞共同培养,使用 MTT法检测活细胞的数量,观察水芹总酚酸对TZM-bl细胞的毒性作用;用人类免疫缺陷病毒1型实验室适应株SF33、BAL分别感染TZM-bl细胞,基于TZM-bl细胞的荧光素酶检测体系,采用荧光素酶活性检测方法检测水芹总酚酸抗人类免疫缺陷病毒1型的活性.结果 水芹总酚酸对TZM-bl细胞表现出较低的细胞毒性,半数细胞毒浓度>1600 μg·ml-1.水芹总酚酸对 TZM-bl细胞 HIV-1 SF33、HIV-1 BAL病毒的半抑制浓度平均值分别为38.93和24.25 μg·ml-1,治疗指数分别为>41和>66.结论 水芹总酚酸在体外具有抑制人类免疫缺陷病毒1型复制活性的作用.
Abstract
Objective To investigate the role of total phenolics acid of Oenanthe Javanica in inhibiting human immunodeficiency virus type 1 in TZM-bl cells.Methods Cultured TZM-bl cells were treated with total phenolics acid extracted from Oenanthe Javanica at different concentrations and the numbers of living cell were calculated using the MTT assay.The anti-human immunodeficiency virus type 1 activity of total phenolics acid extracted from Oenanthe Javanica was assessed using the luciferase detection method.TZM-bl cells were infected with human immunodeficiency virus type 1 laboratory-adapted strains SF33 and BAL respectively and served as the basis for the luciferase detection system.Results The total phenolics acid extracted from Oenanthe Javanica exhibi-ted low toxicity towards TZM-bl cells,with the median cytotoxic concentration exceeding 1600 μg·ml-1.The average half maximal inhibitory concentrations were determined as 38.93 and 24.25 μg·ml-1 for total phenolics acid extracted from Oenanthe Javanica+human immunodeficiency virus type 1 SF33 and total phenolics acid extracted from Oenanthe Javanica+human immunodeficiency virus type 1 BAL groups,respectively.In addition,the therapeutic indexes exceeded 41 and 66,respectively.Conclusion These data suggest that total phenolics acid extracted from Oenanthe Javanica can inhibit human immunodeficiency virus type 1 replication in vitro to some extent.
关键词
水芹总酚酸/人类免疫缺陷病毒1型/抗病毒活性Key words
total phenolics acid extracted from Oenanthe Javanica/human immunodeficiency virus type 1/antiviral activity引用本文复制引用
基金项目
中国医药教育协会2020重大科学攻关问题和医药技术难题科研课题(2020KTZ001)
出版年
2024
NETL
NSTL
万方数据