In Vitro Anti HIV-1 Effect of Total Phenolics Acid Extracted from Oenanthe Javanica
Objective To investigate the role of total phenolics acid of Oenanthe Javanica in inhibiting human immunodeficiency virus type 1 in TZM-bl cells.Methods Cultured TZM-bl cells were treated with total phenolics acid extracted from Oenanthe Javanica at different concentrations and the numbers of living cell were calculated using the MTT assay.The anti-human immunodeficiency virus type 1 activity of total phenolics acid extracted from Oenanthe Javanica was assessed using the luciferase detection method.TZM-bl cells were infected with human immunodeficiency virus type 1 laboratory-adapted strains SF33 and BAL respectively and served as the basis for the luciferase detection system.Results The total phenolics acid extracted from Oenanthe Javanica exhibi-ted low toxicity towards TZM-bl cells,with the median cytotoxic concentration exceeding 1600 μg·ml-1.The average half maximal inhibitory concentrations were determined as 38.93 and 24.25 μg·ml-1 for total phenolics acid extracted from Oenanthe Javanica+human immunodeficiency virus type 1 SF33 and total phenolics acid extracted from Oenanthe Javanica+human immunodeficiency virus type 1 BAL groups,respectively.In addition,the therapeutic indexes exceeded 41 and 66,respectively.Conclusion These data suggest that total phenolics acid extracted from Oenanthe Javanica can inhibit human immunodeficiency virus type 1 replication in vitro to some extent.
total phenolics acid extracted from Oenanthe Javanicahuman immunodeficiency virus type 1antiviral activity
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