The Mechanism of a Traditional Chinese Medicine Enema in the Adjuvant Treatment of Ulcerative Colitis
Objective To investigate the protective and adjuvant therapeutic effects of a traditional Chinese medicine enema preparation against ulcerative colitis in mice based on molecular pharmacology.Methods A mouse model of ulcerative colitis induced by dextran sulfate sodium salt was constructed before the mice were randomly divided into the normal control group,model control group,mesalazine group,and mesalazine + enema solution group.The mice except those in the normal control group drank 4%dextran sulfate sodium salt aqueous solution freely for 7 days.The mesalazine + enema liquid group was given 0.52 g·kg-1 mesalazine by gavage every day,and self-made Chinese medicine preparations were used as an enema each morning and evening.The mesalazine group was given 0.52 g·kg-1 mesalazine by gavage every day,and a normal saline enema each morning and evening.The normal control group and the model control group were gavaged with normal saline every day,plus a normal saline enema every morning and evening for 14 consecutive days before the colonic tissues were frozen for later use.Results Ulcerative colitis could significantly reduce the body weight of mice,which could be reversed by mesalazine,and the effect of the mesalazine + enema liquid group was better than that of the mesalazine group.The results of hematoxylin-eosin staining and histological scoring showed that mesalazine could effectively reduce the histological score of colon tissues in mice,and this effect could be enhanced by the combined use of enema fluid.The contents of TNF-α,INF-γ and IL-6 in mouse colon tissues were detected with ELISA,and the enema fluid could synergistically enhance the down-regulation of mesalazine on inflammatory factors.The expressions of apoptotic genes cleaved caspase-3,Bax and Bcl-2 in mouse colon tissues were detected by Westen blot,and the enema fluid could synergistically enhance the up-regulation of mesalazine on cleaved caspase-3 and Bax proteins and the down-regulation of Bcl-2 proteins.Conclusion The combined use of traditional Chinese medicine enema liquid can synergistically enhance the therapeutic effect of mesalazine against mouse colon tissues,enhance the inhibitory effect of mesalazine on inflammatory factors,and enhance the inhibitory effect of mesalazine on apoptosis.
ulcerative colitistraditional Chinese medicine enemainflammatory responseapopto-sismice
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