Hypoglycemic Activities of Lycium Barbarum Polysaccharide by Immobilized Enzyme Technology
Objective To study the inhibitory effect of Lycium barbarum polysaccharides of different molecular weight segments on α-glucosidase activity and evaluate the hypoglycemic activity.Methods α-glucosidase was immobilized on Fe3O4 magnetic nanomaterials.The content of p-Nitrophenol was determined by HPLC with p-Nitrophenyl-α-D-glucopyranoside as the substrate.The effects of lycium barbarum polysaccharides of different molecular weights on α-glucosidase activity were detected.Results All Lycium barbarum polysaccharides with different molecular weights showed α-glucosidase inhi-bition.Lycium barbarum polysaccharide 3 and lycium barbarum polysaccharides 1 had the best inhibitory effect.When the concentration was 2.5 mg·ml-1,the inhibition rate of Lycium barbarum polysaccharide 3 and Lycium barbarum polysaccharide 1 was 74%and 42%,respectively,which were potential sources of α-glucosidase inhibitors.Conclusion This method can be used to evaluate the inhibitory effect of Lycium barbarum polysaccharides of different molecular weight segments on α-glucosidase activity,which provides evidence for searching for potential hypoglycemic components of Lycium barbarum L.
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