摘要
目的 研究不同分子量段的枸杞多糖对 α-葡萄糖苷酶活性的抑制作用,评价其降血糖活性.方法 利用固定化酶技术,将α-葡萄糖苷酶固定到Fe3O4 磁性纳米材料上,以对硝基苯基-α-D-吡喃葡萄糖苷为底物,通过高效液相色谱法测定酶解产物对硝基苯酚的含量,并计算酶抑制率,检测不同分子量段枸杞多糖对α-葡萄糖苷酶活性的影响.结果 不同分子量段枸杞多糖均表现出一定的 α-葡萄糖苷酶抑制性.其中枸杞多糖3和枸杞多糖1的抑制作用最好,当浓度为2.5 mg·ml-1 时,枸杞多糖3的抑制率可达到74%,枸杞多糖1的抑制率为 42%,是潜在的 α-葡萄糖苷酶抑制剂药物来源.结论 该方法评价了不同分子量段枸杞多糖对 α-葡萄糖苷酶活性的抑制作用,为寻找潜在的枸杞降血糖成分提供了理论参考.
Abstract
Objective To study the inhibitory effect of Lycium barbarum polysaccharides of different molecular weight segments on α-glucosidase activity and evaluate the hypoglycemic activity.Methods α-glucosidase was immobilized on Fe3O4 magnetic nanomaterials.The content of p-Nitrophenol was determined by HPLC with p-Nitrophenyl-α-D-glucopyranoside as the substrate.The effects of lycium barbarum polysaccharides of different molecular weights on α-glucosidase activity were detected.Results All Lycium barbarum polysaccharides with different molecular weights showed α-glucosidase inhi-bition.Lycium barbarum polysaccharide 3 and lycium barbarum polysaccharides 1 had the best inhibitory effect.When the concentration was 2.5 mg·ml-1,the inhibition rate of Lycium barbarum polysaccharide 3 and Lycium barbarum polysaccharide 1 was 74%and 42%,respectively,which were potential sources of α-glucosidase inhibitors.Conclusion This method can be used to evaluate the inhibitory effect of Lycium barbarum polysaccharides of different molecular weight segments on α-glucosidase activity,which provides evidence for searching for potential hypoglycemic components of Lycium barbarum L.
基金项目
国家科技部重点研发计划项目(2018YFC1706305)
甘肃省科技计划项目(21JR1RA271)
2021年度甘肃高等学校产业支撑计划项目(2021CYZC-40)
甘肃省教育厅2023年高校"创新之星"项目(2023CXZX-740)
NETL
NSTL
万方数据