Objective To improve the quality standard of Shensu pills by revising the thin layer chro-matography method for identification of Glycyrrhiza uralensis Fisch and establishing a high performance liquid chromatograph(HPLC)method for quantification of naringin,hesperidin and neohesperidin.Methods Based on the 2020 edition of Chinese Pharmacopoeia,a thin layer chromatography method was established for identification of Glycyrrhiza uralensis Fisch.A HPLC method was also established for quan-tification of naringin,hesperidin and neohesperidin.Results The first method could well identify Glycyrrhiza uralensis Fisch and Platycodon grandiflorum.The calibration curve of naringin was linear over the range of 0.0140-0.2235 mg·ml-1(r=1.0000)and the average recovery of water pills was 97.22%(RSD=0.68%,n=6),compared with 99.43%(RSD=0.32%,n=6)for candied pills.The calibration curve of hesperidin was in good linearity over the range of 0.0114-0.1824 mg·ml-1(r=1.0000)and the average recovery was 95.93%for water pills(RSD=0.48%,n=6),and 96.86%for candied pills(RSD=0.32%,n=6).The calibration curve of neohesperidin was in good linearity over the range of 0.0131-0.2102 mg·ml-1(r=1.0000)and the average recovery of water pills was 100.21%(RSD=0.49%,n=6),and that of candied pills was 97.57%(RSD=0.42%,n=6).Conclusion This method is sensitive and reproducible,which can be used for quality control of Shensu Pills.
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