Objective To optimize the extraction process of Jiahong Shenghua prescription using the Box-Behnken response surface method.Methods Based on the single factor experiment,the solid-liquid ratio,extraction time and number of times of extractions were taken as factors for investigation,while the ferulic content and extract yield were chosen as the evaluation indices to optimize the extraction process with the Box-Behnken response surface method.Results The best extraction process involved a solid-liquid ratio of 1:8,extraction time of 1.5 h,and two-time extraction.Under this condition,the content of feru-lic and the extract yield were 0.584 mg·g-1 and 18.15％respectively.The comprehensive score was 98.13(RSD=0.39％),which was close to the predicted value of 98.92.The standard deviation was 0.80％.Conclusion This extraction process is stable,reliable and highly predictive,which can provide references for the development of traditional Chinese medicine related to Jiahong Shenghua prescription.
关键词
加红生化方/阿魏酸/Box-Behnken响应面法/提取工艺
Key words
Jiahong Shenghua prescription/ferulic/Box-Behnken response surface method/extraction process
维普
万方数据