Objective To establish a quantitative determination method for a hot compressed package of traditional Chinese medicine.Methods The HPLC method was used to determine hydroxysafflor yellow A in the hot compressed package.Chromatographic conditions included Agilent 1260 HPLC,Kromasil C18(4.6 mm×250 mm,5 μm),a mobile phase composed of acetonitrile-methanol-0.7％phosphoric acid solu-tion(2∶26∶72),a velocity of 1.0 ml·min-1,a detection wavelength of 403 nm,and a column tempera-ture of 30 ℃.Results Within the range of 0.511-1.789 μg,the calibration cure was linear(r=0.9999).The mean recovery rate was 98.65％,with an RSD of 0.61％(n=6).The mean content of hydroxysafflor yellow A in the three batches of medicine was 0.83 mg·g-1.Conclusion This determination method is stable,accurate,reproducible,which can be used as a quality detection for hot compressed packages of traditional Chinese medicine.
关键词
中药热敷包/高效液相色谱法/羟基红花黄色素A/含量测定
Key words
hot compressed package of traditional Chinese medicine/HPLC/hydroxysafflor yellow A/content determination
维普
万方数据