Establishment and Evaluation of an Acute Plateau Hypoxic-ischemic Brain Injury Model in SD Rats
Objective To establish a plateau model of hypoxic-ischemic brain injury in SD rats.Methods Sixty SD rats were randomly divided into the normoxic control group and hypoxic-ischemic mod-el group.The normoxic control group was fed on simulated plains(about 800 m),while the hypoxic-ische-mic model group was placed in a low-pressure oxygen chamber,which simulated a 6000 m plateau environ-ment,two hours after ligating the right common carotid artery.After exposures to hypoxia and ischemia,the rats were weighed,the body weight and wet and dry weight of brain tissue were recorded,and the brain water content was calculated.ELISA was used to determine the vascular endothelial growth factor(VEGF),tumor necrosis factor(TNF-α),interleukin 1β(IL-1β)and interleukin 6(IL-6)in brain tissue.HE staining was used to observe the pathological changes of brain tissue.Results Compared with the normoxic control group,there was a significant increase in brain water contents in the hypoxic-ischemic group at 1,3 and 5 d,but there was no significant change in the hypoxic-ischemic group at 7 d.IL-1β,IL-6,VEGF and TNF-α were significantly increased in the hypoxic-ischemic group at 1,3 and 5 d(P<0.01).There was no significant change in TNF-α in the 7 d hypoxic-ischemic group,but IL-1β,IL-6 and VEGF were significantly increased in the 7 d hypoxic-ischemic group.Microscopically,the brain tissue of the normoxic control group was structurally intact,with normal neuronal cell morphology,neatly arranged cells in the hippocampal region and small peripheral gaps in the blood vessels.After treatment,the neuro-nal cells in the brain tissue of rats in the 3 and 5 d groups decreased obviously,some cells appeared vacuo-lated,the cells in the molecular layer and vertebral body layer were edematous,and the perivascular gap increased obviously.A few cells in the 1 d group had edema in the molecular layer and cone layer,and the neuronal cells were arranged neatly,but there was no significantly change in the 7 d group.Conclusion A rat model of high-altitude hypoxic-ischemic brain injury has been established,and the animal model in the hypoxic-ischemic 3 d group is found to be more stable.
NETL
NSTL
万方数据
