Quantitative Determination of Salvianolic Acid B in Xiongtong Granules(Sugar Free)by HPLC
Objective To establish a method for quantitative determination of Xiongtong granules(sugar free).Methods High performance liquid chromatography was used to determine the content of salvianolic acid B in Xiongtong granules(sugar free).The sample was extracted with 80%methanol under ultrasonic management for 30 mins.Chromatographic conditions involved Agilent 1260,Kromasil C18(4.6 mm×250 mm,5 μm);a mobile phase composed of methanol-acetonitrile-formic acid-water(30∶10∶1∶59),a velocity of 1.0 ml·min-1,a detecting wavelength of 286 nm,and a column temperature of 25℃.Results Within the range of 0.40 to 1.40 μg,the calibration cure was linear(r=0.9998).The mean recovery rate was 102.30%,RSD 1.98%.The mean content of salvianolic acid B in the three batches of medicine was 3.27 mg·g-1.Conclusion This determination method is highly stable,accurate,repro-ducible,and can be used as a quality standard for Xiongtong granules(sugar free).
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