HPLC法测定无糖型胸痛颗粒中丹酚酸B的含量
Quantitative Determination of Salvianolic Acid B in Xiongtong Granules(Sugar Free)by HPLC
张丽荣 1彭军 1杨光 1凌云1
作者信息
摘要
目的 建立无糖型胸痛颗粒含量测定方法.方法 采用高效液相色谱法测定无糖型胸痛颗粒中丹酚酸B的含量,样品用80%甲醇超声提取30 min.采用Agilent 1260色谱仪,Kromasil C18 色谱柱(4.6 mm×250 mm,5 μm);流动相:甲醇-乙腈-甲酸-水(30∶10∶1∶59);检测波长:286 nm;柱温:25℃;流速:1.0 ml·min-1.结果 丹酚酸B进样量在0.40～1.40 μg范围内与其峰面积有良好的线性关系,相关系数r=0.999 8,该法平均回收率为102.30%,RSD为1.98%(n=6),三批无糖型胸痛颗粒中丹酚酸B平均含量为3.27 mg·g-1.结论 本法稳定可靠,重现性好,可为无糖型胸痛颗粒质量标准的建立提供参考.
Abstract
Objective To establish a method for quantitative determination of Xiongtong granules(sugar free).Methods High performance liquid chromatography was used to determine the content of salvianolic acid B in Xiongtong granules(sugar free).The sample was extracted with 80%methanol under ultrasonic management for 30 mins.Chromatographic conditions involved Agilent 1260,Kromasil C18(4.6 mm×250 mm,5 μm);a mobile phase composed of methanol-acetonitrile-formic acid-water(30∶10∶1∶59),a velocity of 1.0 ml·min-1,a detecting wavelength of 286 nm,and a column temperature of 25℃.Results Within the range of 0.40 to 1.40 μg,the calibration cure was linear(r=0.9998).The mean recovery rate was 102.30%,RSD 1.98%.The mean content of salvianolic acid B in the three batches of medicine was 3.27 mg·g-1.Conclusion This determination method is highly stable,accurate,repro-ducible,and can be used as a quality standard for Xiongtong granules(sugar free).
关键词
无糖型胸痛颗粒/高效液相色谱法/丹酚酸B/含量测定Key words
Xiongtong granules(sugar free)/HPLC/salvianolic acid B/content determination引用本文复制引用
出版年
2024
NETL
NSTL
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