Content determination of Raw Materials of Furagin by High Performance Liquid Chromatography
Objective To determine the contents of active pharmaceutical ingredients in raw materi-als of furagin by high performance liquid chromatography in order to contribute to quality control.Methods The column was a WondaCract ODS-2(4.6 mm×250 mm,5 μm),the mobile phase was water(pH 4.0,the aqueous phase adjusted by phosphoric acid)-acetonitrile(60∶40),the flow rate was 1.0 ml·min-1,the column temperature 30℃,the detection wavelength 292 nm,the injection volume 20 μl,the running time 30 min,and the duration of equilibration was 20 min.Results The linear range of furagin was 0.1-0.8 mg·ml-1(r=0.9997).Furagin was well separated from its degraded substances in the specificity test.The RSD was 0.46%(n=6)for the instrumental precision test and 0.41%(n=6)for the reproducibility test.The mean recovery was 100.6%with an RSD of 0.97%(n=9).Conclusion This method is simple,sensitive,with good separation and accurate results,which can be used as a method for quality control of active pharmaceutical ingredients in raw materials of furagin.
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