A Tumor-suppressor Mouse Model of BCG for Therapeutic Use and Mechanisms
Objective To investigate the in vivo inhibitory effect of bacillus Calmette-Guérin(BCG)for therapeu-tic use against mouse bladder cancer cells.Methods The bacterial suspension of BCG for therapeutic use was mixed with mouse bladder cancer cell suspension of the same volume before being subcutaneously inoculated with C57BL/6J mice.After 3 weeks,the subcutaneous solid tumors were taken and weighed to calculate the tumor inhibition rate.Meanwhile,the spleens of C57BL/6J mice were taken,the numbers of spots of secreted antigen-specific interferon-γ(IFN-γ)and inter-leukin-2(IL-2)cells were detected using the enzyme linked immunospot assay method.Results The tumors in the BCG for therapeutic use group were much lighter than those in the model control group.The rate of tumor growth inhibition exceeded 90%when BCG for therapeutic use was 10 mg·ml-1 and 5 mg·ml-1 and was above 70%when BCG was 2.5 mg·ml-1,suggesting significant dose dependence.IFN-γ and IL-2 cell spots in this group far outnumbered those of the control group.Conclusion A subcutaneous tumor-suppressor mouse model for BCG for therapeutic use has been estab-lished.The anti-tumor effect may be generated by activating Th1-type immune response and increasing the level of cell secretion of IL-2 and IFN-γ,thus promoting cell-mediated immune response.
mouse bladder cancer cellsBCGC57BL/6J micebladder cancer
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