Research progress of lung organoids in respiratory infectious diseases
Organoids are cell mini-clusters and three-dimensional(3D)micro-organs cultured and spontaneously developed under suspension culture condition in vitro,possessing the abilities of self-organize and differentiate into functional cells.They are powerful,and can partially mimic the cellular heterogeneity,structure and function of the original tissues or organs.Lung organoids(LOs)can be cultured and constructed from human pluripotent stem cells or adult stem/progenitor cells.Co-culture of LOs with immune cells can better reflect the overall picture of the immune response of lung tissue and the full spectrum of infection in vivo.This review compares characteristics of LOs with those common models for respiratory infectious diseases,including animal models,two-dimensional cell culture,lung-on-chip and precision-cut lung slices.We provide an overview of the construction method of LOs and their application progress on respiratory system infectious diseases caused by viruses,bacteria,mycobacteria,cryptosporidium,and other pathogens.
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维普
