减数分裂内切酶1高表达对肝细胞癌预后的影响
Effect of high expression of endonuclease meiotic 1 on the prognosis of hepatocellular carcinoma
王可欣 1陈椿 2贺梦雯 3李乐 4刘妍 4王洪波 5王春艳 5赵景民 6纪冬7
作者信息
- 1. 安徽医科大学解放军307临床学院/第五临床医学院,安徽合肥 230032
- 2. 南方医科大学第二临床医学院,广东广州 510515
- 3. 北京大学302临床医学院,北京 100191
- 4. 解放军总医院第五医学中心感染病医学部,北京 100039
- 5. 解放军总医院第五医学中心肝病医学部,北京 100039
- 6. 解放军总医院第五医学中心病理科,北京 100039
- 7. 安徽医科大学解放军307临床学院/第五临床医学院,安徽合肥 230032;南方医科大学第二临床医学院,广东广州 510515;北京大学302临床医学院,北京 100191;解放军总医院第五医学中心肝病医学部,北京 100039
- 折叠
摘要
目的 探讨减数分裂内切酶1(EME1)的高表达对肝细胞癌(HCC)预后的临床意义.方法 利用癌症和肿瘤基因图谱(TCGA)数据库及高通量基因表达(GEO)数据库,对HCC肿瘤与非肿瘤组织差异表达基因进行筛选并进行生存分析.回顾性收集2010年1月-2014年12月于解放军总医院第五医学中心行肝癌切除术的80例患者的病理组织样本,采用免疫组织化学法检测EME1的表达情况,进行生存分析,评估EME1对肝癌患者术后5年生存率的影响;采用基因富集分析预测EME1在HCC中的功能.结果 TCGA数据库筛选出371例HCC癌组织及50例非肿瘤组织样本,分析显示EME1 mRNA在HCC癌组织中明显高表达.筛选GEO数据库中的107例样本(包括70例HCC癌组织,37例非肿瘤组织),癌组织的EME1 mRNA表达量明显高于非肿瘤组织(P<0.05).生存分析显示EME1高表达组术后5年总体生存率明显低于低表达组(44.1%vs.53.0%,P<0.05).免疫组化结果半定量分析显示,EME1高表达组的总体生存率明显低于低表达组(32.8%vs.45.0%,P<0.05),多因素COX分析显示,EME1高表达[风险比(HR)=2.234,95%CI 1.073～4.649,P=0.032]和中国肝癌分期(CNLC)高分期(HR=4.317,95%CI 1.799～10.359,P=0.001)是影响HCC患者术后5年生存率的独立危险因素.结论 EME1在HCC组织中高表达,并与HCC患者的不良预后相关,可作为HCC治疗的潜在靶点.
Abstract
Objective To elucidate the clinical significance of high expression levels of endonuclease meiosis 1(EME1)in the prognosis of hepatocellular carcinoma(HCC).Methods The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases were used to screen and analyze differential gene expression between HCC and non-tumor tissues.A retrospective collection of liver tissue samples from 80 HCC patients who underwent hepatectomy in the Fifth Medical Center of Chinese PLA General Hospital between January 2010 and December 2014 was performed.Immunohistochemistry analysis was employed to detect the EME1 expression levels.Survival analysis was then conducted to assess the impact of EME1 expression on 5-year postoperative survival rate of HCC patients.Additionally,gene enrichment analysis was applied to predict the function of EME1 in HCC.Results A total of 371 HCC tissue samples and 50 non-tumor liver tissue samples from TCGA database were analyzed,revealing significantly higher EME1 expression in HCC tissues.Microarray analysis of 107 samples within the GEO database(70 HCC tissues and 37 non-tumor tissues)confirmed that EME1 mRNA expression was markedly elevated in HCC tissues compared with non-tumor tissues(P<0.05).The 5-year overall survival(OS)rate was notably lower in high EME1 expression group than that in low expression group(44.1%vs.53.0%,P<0.05).Semi-quantitative immunohistochemistry analysis demonstrated that patients with high EME1 expression had a significantly lower OS rate than those with low EME1 expression(32.8%vs.45.0%,P<0.05).Multivariate COX regression analysis identified that high EME1 expression(HR=2.234,95%CI 1.073-4.649,P=0.032)and advanced China liver caner(CNLC)staging(HR=4.317,95%CI 1.799-10.359,P=0.001)were independent risk factors for the 5-year OS of post-operation patients with HCC.Conclusion Elevated EME1 expression in HCC tissues correlates with an adverse prognosis of HCC and suggests that EME1 could serve as a potential therapeutic target for HCC.
关键词
肝细胞癌/减数分裂内切酶1/免疫组化/生存分析Key words
hepatocellular carcinoma/endonuclease meiotic 1/immunohistochemistry/survival analysis引用本文复制引用
基金项目
北京市自然科学基金面上项目(7222173)
出版年
2024
国家科技期刊平台
NSTL
万方数据