橘皮素缓解APP/PS1小鼠小胶质细胞过度激活及认知功能障碍
Tangeretin Alleviates Microglia Over-activation and Cognitive Impairment in APP/PS1 Mice
于晶 1梁征 1贺子珊 1陈鸿霖 1石成英 1龚晓康 1包建 1舒细记1
作者信息
- 1. 江汉大学 武汉生物医学研究院,湖北 武汉 430056
- 折叠
摘要
目 的 观察橘皮素(tangeretin,TAN)对APP/PS1小鼠认知功能,以及脑内小胶质细胞过度激活和炎症因子分泌的影响.方 法 2.5月龄雄性APP/PS1小鼠,通过口服给予TAN,连续干预 16周,观察TAN对缓解APP/PS1认知功能障碍,以及脑内小胶质细胞过度激活和炎症因子分泌的作用.结 果 通过行为学检测发现TAN明显改善APP/PS1小鼠认知障碍;通过免疫荧光染色,发现TAN显著缓解APP/PS1小鼠脑内小胶质细胞的过度激活;多因子分析检测发现,TAN显著升高小鼠脑内抑炎因子IL-2和IL-5的水平.结 论 TAN显著减轻APP/PS1小鼠脑内小胶质细胞过度激活以及调节炎症因子分泌,缓解小鼠的认知障碍.
Abstract
Objective To study the effects of tangeretin(TAN)on cognitive performance,microglia over-activation,and inflammatory factors released in APP/PS1 mice.Methods 2.5-month-old male APP/PS1 mice were treated with TAN orally for 16 weeks.The effects of TAN on alleviating cognitive dysfunction of APP/PS1,microglia over-activation,and inflammatory factors released in the brain were observed.Results It was found that TAN significantly alleviated cognitive impairment in APP/PS1 mice by behavioral testing;the results of immunofluorescence staining demonstrated that TAN significantly alleviated microglia over-activation in the brain of APP/PS1 mice;the results of multifactor analysis showed that TAN significantly increased the levels of anti-inflammatory molecules IL-2 and IL-5 in the mouse brain.Conclusion TAN significantly reduces microglia over-activation,regulates the release of inflammatory factors in the brain of APP/PS1 mice,and alleviates cognitive impairment.
关键词
阿尔兹海默症/橘皮素/小胶质细胞/炎症因子Key words
Alzheimer's disease/tangeretin/microglia/inflammatory factors引用本文复制引用
基金项目
湖北省自然科学基金面上项目(2022CFB456)
出版年
2024
国家科技期刊平台
NSTL
万方数据