瘤胃纤毛虫体外培养技术研究进展
Research Progress of in vitro Culture Technology of Rumen Ciliates
徐勤辉 1何金英 1陆源鸿 1彭成 1鲁小悦 1杨紫贤 1熊杰 2姜传奇 2缪炜 2冯金梅1
作者信息
- 1. 江汉大学 医学部病原生物学教研室,湖北 武汉 430056
- 2. 中国科学院 水生生物研究所水生生物多样性与保护重点实验室,湖北 武汉 430072
- 折叠
摘要
瘤胃纤毛虫是一类主要生活在反刍动物瘤胃内的单细胞真核微生物,可能在瘤胃高效降解植物纤维、调节瘤胃发酵速率和参与瘤胃温室气体甲烷产生等方面具有重要作用.深入探究瘤胃纤毛虫的功能及其对宿主的影响,实现其体外培养是一项不可或缺的技术手段.综述了瘤胃纤毛虫体外培养技术研究进展,首先介绍了瘤胃纤毛虫体外培养的国内外研究现状,然后概述了影响瘤胃纤毛虫体外培养的因素,最后指出建立的瘤胃纤毛虫体外培养体系的应用及前景,为建立稳定可控的瘤胃纤毛虫体外单种生长与增殖培养体系提供参考.
Abstract
Rumen ciliates are a group of single-celled eukaryotic microorganisms mainly living in the rumen of ruminants,which may play essential roles in the efficient degradation of plant fibers,regulation of rumen fermentation rate,and participation in the rumen methanogenesis.To deeply research the functions of rumen ciliates and their impact on the host,it is necessary to culture them in vitro.Here,we reviewed the research on the progress of culture technology of rumen ciliates in vitro.Firstly,we introduced the global research status of the culture of rumen ciliates in vitro,then summarized the factors that influence it,and finally,proposed applying the established culture system of rumen ciliates in vitro.This review provides a valuable reference for establishing a stable and controllable culture system of rumen ciliates in vitro.
关键词
真核微生物/瘤胃纤毛虫/体外培养/厌氧培养Key words
eukaryotic microorganisms/rumen ciliates/in vitro cultivation/anaerobic culture引用本文复制引用
基金项目
国家自然科学基金面上项目(32070420)
国家重点研发计划青年科学家项目(2022YFF1001100)
出版年
2024
国家科技期刊平台
NSTL
万方数据