Yishen Jianpi Huayu Decoction mitigated STZ induced kidney injury in rats by regulating the Keap1/Nrf2/HO-1 pathway
Objective To observe the reduction of STZ-induced kidney injury in rats by Yishen Jianpi Huayu Decoction(YJHD)based on Keap1/Nrf2/HO-1 pathway.Method Ten of 60 SD male rats were randomly selected as the control group and fed with normal maintenance chow,while the remaining 50 rats were fed with high-fat chow,and after 4 weeks,a single low-dose intraperitoneal injection of streptozotocin(STZ)was administered,40 successfully modeled rats were randomly divided into 4 groups according to 10 rats in each group:model group,low group,high dose administration group and positive drug group[Cagliflozin(CANA)group],and the positive drug was chosen as Cagliflozin(CANA),which was gavaged for 8 weeks,while the model group was gavaged with an equal volume of distilled water during the same time.Oral glucose tolerance(OGTT)as well as area under the curve(AUC)were measured after drug administration,urine was collected to measure 24-hour urine protein,blood was taken at the time of sampling,and serum samples were used to measure blood creatinine(Cre),blood urea nitrogen(BUN),and levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT),and malondialdehyde(MDA).HE staining was performed to observe the pathological changes in renal tissues,and Western blot was performed to detect the levels of Keap1,Nrf2,and HO-1 in renal tissues.Results Compared with the con-trol group,FBG and AUC were significantly increased(P<0.05),24-hour urine protein,Cre and BUN levels were significantly in-creased(P<0.05),MDA was increased,SOD,GSH-Px and CAT were decreased(P<0.05),a large number of inflammatory cell pro-liferation infiltration was seen in kidney tissues,and the expression of Keap1 was increased,Nrf2 and HO-1 expression decreased(P<0.05);compared with the positive drug group,FBG and AUC levels of YJHD group were seen to decrease(P<0.05),24-hour urine protein,Cre and BUN levels were significantly improved(P<0.05),MDA levels decreased,and SOD,GSH-Px and CAT levels in-creased(P<0.05),while in the renal tissue,the cell proliferation infiltration was alleviated,the protein expression of Keap1 was re-duced,and the protein expression of Nrf2 and HO-1 was significantly increased(P<0.05).Conclusion Yishen Jianpi Huayu Decoction(YJHD)may alleviate and mitigate STZ-induced kidney injury in rats through the Keap1/Nrf2/HO-1 pathway.
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