The significance of quantitative analysis of DNA ploidy binding P16 protein in cervical cancer screening
Objective To investigate the significance of DNA quantitative analysis combined with immunohistochemical staining of P16 proteinin in cervical cancer screening.Method A total of 4 334 outpatient and inpatient patients were selected as research sub-jects.For individuals with abnormal DNA quantitative analysis in cervical cytology,with informed consent,cervical biopsy,histopatho-logical examination,and P16 protein staining examination under colposcopy will be performed to compare and analyze the relationship between DNA ploidy quantitative analysis of abnormal individuals and their histological pathology,as well as the relationship between P16 protein expression and pathological histology,in order to evaluate the significance of combining DNA quantitative analysis with P16 staining in cervical cancer screening.Results Among 4 334 patients,369 were found to have abnormal ploidy.Colposcopic biopsy was recommended for 202 cases of abnormal ploidy,and 150 cases were found to be above CINⅠ.Meanwhile,P16 protein staining was performed for 202 cases ofabnormal ploidy,and it was found that the percentage of P16 expression increased with the increase of lesion grade.The expression of P16 protein in cervical intraepithelial neoplasia was statistically significant by Chisquare test(P<0.05).Con-clusion Quantitative analysis of DNA ploidy has certain significance in cervical cancer screening,and combined with immunohisto-chemical staining of P16 protein and cervical biopsy,has significance in detecting early cervical lesions.
DNA ploidy quantitative analysisP16 proteinImmunohistochemistryCervical cancer
万方数据
维普
