Correlation between serum levels of Zonulin and Claudin-5 and depressive behavior in rats with post stroke depression
Objective To explore the correlation between serum Zonulin and Claudin-5 levels and depression-like behavior in post-stroke depressed rats,and to provide evidence for the diagnosis and treatment of post-stroke depression and the evaluation of therapeutic effect.Method The SD rat model of post-stroke depression was established by MCAO combined with CUMS.The symptoms of anhedo-nia and depression were measured by sucrose water preference test and forced swimming test.Open field test was used to observe the autonomic motor ability,anxiety and depression behavior of the two groups of rats.Serum levels of Zonulin and Claudin-5 were deter-mined by enzyme-linked immunosorbent assay(ELISA).Logistic regression was used to analyze the correlation between serum Zonulin and Claudin-5 levels and depression-like behavior in post-stroke depressed rats.To evaluate the value of serum Zonulin and Claudin-5 levels in early diagnosis and efficacy evaluation of post-stroke depression.Results Compared with the control group,the rats in the post-stroke depression group showed significantly decreased sucrose water preference rate,significantly prolonged forced swimming time and other depression-like behaviors,and the serum levels of Zonulin and Claudin-5 were significantly increased.Logistic regression a-nalysis showed that serum levels of Zonulin and Claudin-5 were negatively correlated with rats' sucrose water preference rate,and posi-tively correlated with rats' forced swimming ignorance of time.Conclusion Serum levels of Zonulin and Claudin-5 can reflect the severi-ty of post-stroke depression-like behavior in rats to a certain extent,and may have potential value for early diagnosis and therapeutic e-valuation of post-stroke depression.
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