The method of detecting fluorescein-labeled tuberculosis by flow cytometry
Objective To establish a flow cytometry method for detecting fluorescein-labeled mycobacterium tuberculosis(M.tb).Method M.tb was labeled with Fluorescein Isothiocyanate,Fluorescein Diacetate,PKH26 and Propidium Iodide.The labeling rate of M.tb was detected by flow cytometry.Results M.tb was labeled with FITC,and there were significant differences in the labeling yield of different concentrations of FITC in PBS buffer,the labeling rate of FITC at 10 μg/ml was 85%,and the labeling rate of FITC at 40 μg/ml was 93%,and the labeling rate increased with the increase of concentration(P<0.05);In CB buffer,the labeling rate of FITC-labeled M.tb with different concentrations was about 90%,and there was no significant difference(P>0.05).The labeling rate of FDA labeled M.tb was more than 92%.The labeling rate of M.tb labeled by PKH26 was more than 90%.The labeling rate of M.tb was 47%after 1min of alcohol treatment,and 97%after 2min of alcohol treatment.The labeling rate increased with time(P<0.05).Conclusion The establishment of a flow cytometry method for the detection of fluorescein-labeled M.tb can provide experi-mental ideas for the basic research of tuberculosis.
万方数据
维普
