Objective To investigate the effects of anwulignan on D-galactose-induced learning and memory impairment in mice and its relation to apoptosis.Method Male ICR mice were randomly divided into 5 groups,namely,control group(gavage of sodium carboxy-methyl cellulose solution,subcutaneous injection of normal saline),model group(gavage of sodium carboxymethyl cellulose solution,subcutaneous injection of 220 mg/kg D-galactose),and low,medium and high dose group(gavage of 1,2,and 4 mg/kg of anwulignan,subcutaneous injection of 220 mg/kg D-galactose).All mice were administered continuously for 42 days,once a day.The learning and memory abilities of mice were analyzed with step-down tests.After the behavioral experiment,the expressions of apoptosis-related gene P53,Bcl2,Bax and Caspase3 in mouse brain hippocampus tissue were detected.Results Compared with the control group,in the model group,the latency of step-down significantly decreased,observed by step-down and Morris water maze tests.After the behavioral tests,the expression of apoptosis-related genes P53,Bcl2,Bax and Caspase3 was detected by Western blot.Results The step-down the number of errors increased significantly(P<0.01),the hippocampus tissue P53 and Caspase3 expression levels increased significantly(P<0.05 or P<0.01),and the Bcl2/Bax ratio decreased significantly(P<0.05 or 0.01).However,compared with the model group,in anwulig-nan group,the latency of step-down significantly prolonged,the number of errors decreased significantly(P<0.01),the hippocampus tis-sue P53 and Caspase3 expression levels decreased significantly(P<0.05 or 0.01),and the Bcl2/Bax ratio increased significantly(P<0.05 or 0.01).Conclusion Anwulignan can improve the learning and memory impairment induced by D-galactose in mice,which may be related to its inhibition of apoptosis in the hippocampus cells of mice.
维普
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