摘要
目的:构建大鼠再生障碍性贫血(AA)模型,探究蒙药益髓补血丸对于AA的造血功能和免疫功能调节作用.方法:将SPF级SD大鼠(200±10)g 60 只,随机分为正常对照组、AA模型组、司坦唑醇治疗组和大、中、小剂量益髓补血丸组,每组 10 只.药物干预组于实验第 14 天开始灌胃给药,第 6 周末处死动物.进行外周血细胞和骨髓有核细胞计数,骨髓病理学检查、脏器指数计算,并采用 ELISA检测炎性因子.结果:AA模型组大鼠外周血三系、粒细胞集落刺激因子(G-CSF)和粒细胞巨噬细胞集落刺激因子(M-CSF)均明显减少,骨髓有核细胞(BMCs)数量降低显著,肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ和白细胞介素(IL)-2和脾脏指数增加、胸腺指数和IL-10 水平降低.经蒙药益髓补血丸治疗能显著改善AA骨髓造血结构破坏,增加骨髓有核细胞、外周血三系细胞数量;有效降低血清TNF-α、IFN-γ和IL-2 炎性因子水平和脾脏指数,提高IL-10 水平和胸腺指数.结论:蒙药益髓补血丸能有效促进AA大鼠骨髓造血,调节免疫功能紊乱,提高炎性因子水平.
Abstract
Objective This study aims to investigate the hematopoietic and immune regulatory effects of Mongolian medicine Yishui Buxue Wan on AA by constructing a rat model of aplastic anemia(AA).Method Sixty SPF grade SD rats(200±10g)were randomly divided into a normal control group,a relapse model group,a stanozolol treatment group,and large,medium,and small dose Yimui Bux-ue Wan groups,with 10 rats in each group.The drug intervention group started administering medication by gavage on the 14th day of the experiment,and the animals were euthanized at the end of the 6th week.Perform peripheral blood cell and bone marrow nucleated cell counts,bone marrow pathology examination,organ index calculation,and detect relevant inflammatory factors using ELISA meth-od.Results The peripheral blood triad,granulocyte colony-stimulating factor(G-CSF),and granulocyte macrophage colony-stimulating factor(M-CSF)were significantly reduced in the aplastic anemia model group of rats,and the number of bone marrow nucleated cells(BMCs)was significantly reduced.Inflammatory factors(TNF-α,IFN-γ,and IL-2)and spleen index increased.The thymus index and IL-10 levels decreased.The treatment with Mongolian medicine Yimui Buxue Wan can significantly improve the destruction of hemato-poietic structure in AA bone marrow,increase the number of nucleated cells in bone marrow and peripheral blood three line cells;Ef-fectively reduce the levels of serum TNF-α,IFN-γ,IL-2 inflammatory factors and spleen index,and increase IL-10 levels and thymus index.Conclusion The Mongolian medicine Yishui Buxue Wan can effectively promote bone marrow hematopoiesis in rats with aplastic anemia,regulate immune dysfunction,and increase cytokine levels.
基金项目
辽宁省教育厅资助课题(JYTMS20231717)
维普
万方数据