Study on the Immunomodulatory Activity of Rheum palmatum on the Intestinal Mucosal Barrier in a Mouse Model of Psoriasis Vulgaris
Objective To investigate the immunoregulatory effects of Rheum palmatum extract on the intestinal barrier function in a mouse model of psoriasis vulgaris,providing new insights and theoretical support for the treatment of psoriasis with traditional Chinese medicine.Method This study established a mouse model of psoriasis vulgaris using 5%clobetasol propionate cream to induce skin le-sions.The experiment was divided into a normal group,a blank group,a positive drug control group,and a rhubarb intervention group,with 15 rats in each group.Except for the normal group,the other three groups were given 100 mg/dose of imiquimod for back application for 8 consecutive days.From the third day of application,patients with silver filings were given fecal water enema.After daily modeling,the positive drug control group was given methotrexate 0.1 mg/ml,and the rhubarb intervention group was given rhu-barb extract 11mg by gavage once a day for 10 consecutive days.The PASI scoring standard is used to score skin lesions,and the ELISA method is used to detect the levels of IL-17 and IL-23 in the intestinal mucosa.Results The PASI scoring results showed that,except for the normal group,the skin of the mice began to show slight damage on the second day.On the 10th day,the degree of skin lesions in mice reached its peak,with the blank group mice showing the most severe skin lesions.Compared with the blank group,the skin scales of mice in the rhubarb intervention group and the positive drug control group were reduced,the skin was smoother,and the PASI score was significantly lower than that of the blank group,with statistical significance(P<0.05).There was no statistically sig-nificant difference in the skin lesions between the rhubarb intervention group and the positive drug control group(P>0.05).The ELISA results showed that compared with the blank group,the levels of IL-17 and IL-23 in the rhubarb intervention group and the posi-tive drug control group mice were significantly reduced,and the difference was statistically significant(P<0.01).Conclusion Rheum palmatum may be one of the mechanisms for treating psoriasis by regulating the expression of IL-17 and IL-23 in intestinal mucosal tis-sue,alleviating inflammatory reactions in mouse intestinal mucosal tissue,and reducing the severity of skin lesions induced by imi-quimod in psoriasis mice.
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