Changes and significance of the expression of SCF and c-Kit in mice model of cryptorchidism
Objective To observe the change expression of the stem cell factor(SCF)and c-Kit in the mice testis tissue of cryp-torchidism model.Method The pregnancy BALB/c mice were divided into five groups A,B,C,D and E randomly;Flutamide was con-secutive gavage administrated at 0 mg/kg、150 mg/kg、300 mg/kg、500 mg/kg、700 mg/kg from gestation day 12 to 21.Detected the relative quantity of testicular SCF mRNA by Real-time PCR And then the expression of c-Kit in the testis by quantum dots technology on postnatal eight weeks.Results The cryptorchidism model of mice was induced successfully.The relative quantity of testicular SCF mRNA in group A,B,C,D,E was 1.00±0.01、0.78±0.04、0.61±0.03、0.45±0.05、0.35±0.03 respectively,and they reduced succes-sively(P<0.05).The expression of c-Kit in the experimental group was much less than in control groups.Conclusion The decreased expression of SCF mRNA and c-Kit may be one of the important reasons of which the testicular spermatogenetic function was damaged in the mice cryptorchidism model induced by Flutamide.
FlutamideStem cell factor(SCF)c-KitCryptorchidismSpermatogenetic function
万方数据
维普
