新扶正除疫颗粒剂对感染腺病毒小鼠肺部凋亡因子的影响

Effect of New Fuzheng Chuyi Granules on Apoptosis Factors in the Lungs of Mice Infected with Adenovirus

刚晓超 ¹宫晓燕 ²岳冬辉 ¹李丽杰 ³慕天一 ³地力诺尔艾·巴拉提 ¹黄若萱 ¹代冰 ¹苑心怡¹

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作者信息

1. 长春中医药大学,吉林长春 130117
2. 长春中医药大学附属医院
3. 长春中医药大学附属第三临床医院
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摘要

目的:探讨不同浓度新扶正除疫颗粒剂干预治疗感染腺病毒小鼠过程中,其肺内凋亡因子天冬氨酸蛋白水解酶-9(Caspase-9)变化情况,并探讨相关机制.方法:将 120 只BALB/c小鼠进行分组,包括低、中、高剂量组、腺病毒模型组与利巴韦林组.动物给药 7 d,最后 1 d后取其肺脏,用流式细胞仪测定Caspase-9肺内凋亡表达水平.结果:各治疗组动物肺组织中的Caspase-9含量与模型组比较,在使用新抗疫颗粒剂治疗后均有显著增加.利巴韦林组与高剂量组的Caspase-9含量比较,差异有统计学意义(P＜0.05).结论:新扶正除疫颗粒剂促Caspase-9 释放,高剂量组与西药组比较,Caspase-9 释放效果更明显.

Abstract

Objective To investigate the changes of apoptosis factor Caspase-9 in the lungs of adenovirus-infected mice treated with New Fuzheng Chuyi Granules,and to explore the related mechanism.Method A total of 120 BALB/c mice were divided into groups,including low,medium and high dose groups,adenovirus model group and western medicine group.The animals were treated for 7 days,and after the last 1 day,their lungs were taken and the expression level of aspartate proteolytic enzyme-9(Caspase-9),that is,apoptosis in the lungs,was determined by flow cytometry.Results The results showed that the content of Caspase-9 in the lung tissues of the treated animals in each treatment group increased significantly compared with the model group after treatment with the New Fuzheng Chuyi Granules.There was a significant difference in Caspase-9 content between the ribavirin group and the high-dose group(P＜0.05).Conclusion The New Fuzheng Chuyi Granules promotes the release of Caspase-9,and the release effect of Caspase-9 is obvious in the high-dose group and the Western medicine group.

关键词

新扶正除疫颗粒剂/腺病毒/凋亡因子/Caspase-9

Key words

New Fuzheng Chuyi Granules/Adenovirus/Apoptotic factors/Caspase-9

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出版年

2025

吉林医学

吉林省人民医院

吉林医学

影响因子：0.926

ISSN：1004-0412

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