Objective To investigate the effect of Yuanluo Tongjing acupuncture on memory impairment of Alzheimer's disease(AD)model mice based on hippocampal acetylated proteomics.Method 30 cases of healthy male Kunming breed mice were divided into blank group,model group and acupuncture group with 10 mice in each group according to random number table method.Mice were intraperitoneally injected with scopolamine solution[100 mg/(kg·d)]in model group and acupuncture group,while mice were intrap-eritoneally injected with the same amount of normal saline in blank group,continuous injection for 7 days to moldel.The mice were needled with the original collateral-Tongjing acupuncture method(once/d)in the acupuncture group,the mice were caught at the same time and degree in the blank group and the model group were,and the intervention lasted for 30 days.Morris water maze behav-ior test and new object recognition test were used to detect the memory ability of mice.HE staining was used to observe the pathological injury of mice hippocampus.The liquid chromatography-tandem mass spectrometry(LC MS/MS)was used to determine acetylated modified proteins from mouse hippocampal tissues and analyze their biological information.Results Compared with the model group,the escape latency period and escape path length of the acupuncture group were significantly shortened,the residence time in the sec-ond quadrant was significantly increased,the number of times through the original platform position was significantly increased,and the recognition index was significantly increased in the acupuncture group(P＜0.05).Compared with the model group,the pathological damage and apoptosis of hippocampus in the acupuncture group were significantly reduced in the acupuncture group.LC MS/MS analy-sis showed that compared with blank group and model group,the overall level of acetylation modification increased in the acupuncture group.Five key hub proteins were identified by acetylation protein interaction network analysis as Hspa8,Rab7a,Nsf,Ezr and Cfl1.The five key hub proteins were not acetylated modification in the model group,while the five key hub proteins were acetylated modifica-tion in the acupuncture group.Conclusion Yuanluo Tongjing acupuncture could effectively improve memory impairment in dementia model mice,reduce the pathological damage in hippocampus,which might be related to acetylated modification of key hub protein in hippocampus.
维普
万方数据
