Objective To explore the influence of the METTL3 on proliferation and migration of murine SCAPs.Method Mouse SCAPs were isolated and cultured;We used the flow cytometry to identify the stem cell surface marker molecules.Alizarin red staining and Oil red O staining were used to identify the multiple differentiation potential of SCAPs.The METTL3 of SCAPs was knocked down by shRNA.Cell proliferation ability and migration capacity were checked by CCK-8 assay,wound healing test and transwell assay.changes in migration ability.Results The cells expressed stem cell surface markers highly which were found by Flow cytometry showed.After mineralization-inducing and adipogenic inducing,The cells counld differentiate into osteo/odontogenic and adipogenic cell.The METTL3 of SCAPs was successfully knocked down by shRNA transfection(P＜0.05).CCK-8 showed that the MET-TL3knockdown in SCAPs reduced proliferative ability(P＜0.05).The migratory ability of SCAPs was inhibited after knocking down METTL3 in SCAPs which were identified by wound healing test and transwell assay(P＜0.05).Conclusion METTL3 plays a facilita-ting role in the proliferation and migration ability of SCAPs.
维普
万方数据