首页|1株鹿源布鲁氏菌分离鉴定及遗传进化分析

1株鹿源布鲁氏菌分离鉴定及遗传进化分析

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梅花鹿是我国重要的名贵药用动物,具有较高的药用价值,用途广泛,在大健康产业中具有重要地位.然而,布鲁氏菌病制约了梅花鹿产业的发展.试验从梅花鹿流产胎中分离到1株布鲁氏菌(Brucella),采用常规及分子分型方法进行鉴定.结果表明:该分离菌株为羊种1型;用MLVA-16方法对分离菌株进行分类及遗传进化分析,结果显示其与地中海型亲缘关系更近,但有4~5个位点不同于其他菌株,表明此鹿源布鲁氏菌分离株可能存在基因变异.试验为鹿布鲁氏菌病的主要流行菌株提供了参考,同时为揭示布鲁氏菌的跨种传播机制提供了新线索.
Isolation and Identification of A Strain of Brucella of Deer Origin and Genetic Evolutionary Analysis
The sika deer is an important and valuable medicinal animal resoure in China,and its whole body has medicinal value and a wide range of uses to make it an important part of the big health industry.However,the prevalence of brucellosis has become one of the key challenges limiting the development of the sika deer industry.In this study,a Brucella strain was isolated from the aborted fetus of a doe of sika deer,which was identified by conventional and molecular typing methods,and the results indicated that the isolate was of Brucella melitensis bv.1.The MLVA-16 method was used to classify and genetically evolve the isolate,and the results showed that it was more closely related to the Mediterranean type,but there were still 4-5 loci different from other strains,which indicated that there might be genetic variation in this deer-derived Brucella isolate.This study provides a reference for clarifying the main prevalent strains of brucellosis in deer,and also provides new clues for uncovering the mechanism of cross-species transmission of Brucella.

deerBrucella of deer originisolation and identificationgenetic evolutionary analysis

李连民、石俊峰、李婷、刘菲、李健明、杜锐

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吉林农业大学动物科学技术学院,长春 130118

吉林农业大学中药材学院,长春 130118

动物生产及产品质量安全教育部重点实验室,长春 130118

吉林省梅花鹿高效养殖和产品开发技术工程研究中心,长春 130118

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梅花鹿 鹿源布鲁氏菌 分离鉴定 遗传进化分析

吉林省科技发展计划技术攻关项目吉林省自然科学基金项目

20190304004YYYDZJ202201ZYTS444

2024

经济动物学报
吉林农业大学

经济动物学报

CSTPCD
影响因子:0.822
ISSN:1007-7448
年,卷(期):2024.28(1)
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