鸭绿江细鳞鲑的DNA条形码及鉴定分析

DNA Barcode and Identification Analysis of Brachymystax lenok in Yalu River

黄权 ¹蒋焯¹

扫码查看

作者信息

1. 吉林农业大学动物科学技术学院,长春 130118
折叠

摘要

应用DNA条形码技术对鸭绿江分布的细鳞鲑样品进行种类鉴定.通过对鸭绿江细鳞鲑的线粒体细胞色素氧化酶 Ⅰ亚基(COⅠ)基因序列进行分析,应用DNA条形码技术鉴定鸭绿江细鳞鲑的种类.结果表明:细鳞鲑线粒体CO Ⅰ基因序列长为699bp,其中G含量较低,A+T含量(52.79％)高于C+G含量(47.21％).细鳞鲑种内差异不明显,样品鱼种内遗传距离均＜0.02.对样品鱼进行DNA基因序列分析,GenBank和Bold Systems搜索结果相似性均＞99.0％.因此,鸭绿江所采样品鱼鉴定为细鳞鲑(Brachymystax lenok).

Abstract

The purpose of this study was to identify the species of Lepidosoma in Yalu River by DNA barcoding technique.Through analyzing the gene sequence of cytochrome oxidase Ⅰ subunit(CO Ⅰ)of Yalu River Lepidopterus,the species of Yalu River Lepidopterus was identified by DNA barcoding.The results showed that the length of mitochondrial CO Ⅰ gene was 699 bp,in which the content of G was lower,the content of A+T(52.79％)was higher than that of C+G(47.21％).There were no significant intraspecific differences and the average intraspecific genetic distance of the samples was less than 0.02.DNA sequence analysis of the fish samples showed that the similarity rate with the GenBank and Bold Systems were all above 99.0％.Therefore,the fish sampled from the Yalu River were identified as Brachymystax lenok.

关键词

细鳞鲑/CO/Ⅰ基因/DNA条形码/鸭绿江

Key words

Brachymystax lenok/CO Ⅰ gene/DNA barcode/Yalu River

引用本文复制引用

基金项目

吉林省自然科学基金项目(20170101026JC)

出版年

2024

经济动物学报

吉林农业大学

经济动物学报

CSTPCD

影响因子：0.822

ISSN：1007-7448

参考文献量10

段落导航