Lactic acid bacteria are important probiotics in the digestive tract of human and animals,and have adjuvant effect and immune regulation function.Therefore,it is of great significance and broad application prospect to use lactic acid bacteria as live vaccine vector to express foreign proteins to develop oral vaccines.In this paper,we mainly introduce the transformation methods of using CR1SPR/Cas9 system for gene editing of lactic acid bacteria,anchored expression system of lactic acid bacteria,invasive lactic acid bacteria expressing FnBPA,DCS targeted lactic acid bacteria and non resistant screening lactic acid bacteria as oral mucosal immune vaccine vectors in order to provide reference for the development of lactic acid bacteria vector vaccine,and analyze and prospect its application prospects.
维普
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