Identification,Genomic and Pathogenicity of a Wild Strain of Mycoplasma gallisepticum in Guizhou Province
The purpose of this study was to investigate the effect of Mycoplsma gallisepticum(MG)infection on lung damage of chickens in Guizhou province.PCR identification was performed on the isolated MG using 16S rRNA primers.The gene sequence was compared with multiple databases such as COG,NR,Swissprot,TrEMBL,etc.through 16S rRNA gene sequencing to obtain functional annotation information.Ten healthy 22-day-old SPF chickens were randomly divided into a control group and a MG infection group,MG infection group chickens were subcutaneously injected with MG into the thoracic cavity of bacterial solution,control group chickens were subcutaneously injected with an equal amount of PBS at the same site for 21 consecutive days.Lung tissue specimens were collected from chickens to observe and record their clinical pathological changes,pathological changes through hematoxylin eosin(HE)staining,and ultrastructural changes through transmission electron microscopy.The results showed that specific nucleotide bands could be amplified,and this wild strain had a high homology of 99.9％with B726-06,B2771-14-1A,and B1 102-06.The phylogenetic tree found that the MG belonged to the Mycoplasma genus of the Mycoplasma family in the Molluscales order,which proved that this wild strain was MG.Animal experiments found that MG infection in chickens could lead to extensive infiltration of inflammatory cells in the lungs,thickening of the lung chamber wall,and pathological changes in capillaries filled with red blood cells.It could also cause cell swelling,abnormal nuclear pyknosis,mitochondrial swelling,cristae rupture,and other ultrastructural changes,which indicated that MG had strong pathogenicity to chickens.This study enriched the species of local avian mycoplasma in China by identifying the wild strain MG in Guizhou province,analyzing its genome and pathogenicity,and provided a basis for the prevention and control of mycoplasma diseases.
国家科技期刊平台
NSTL
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