Comparison of Blood Transcriptome among Sika Deer,Reindeer and Père David's Deer
This study was aimed to explore the differences in gene expression patterns among sika deer,reindeer and Père David's deer.In the experiment,five 3-year-old,healthy male sika deer,reindeer,and Père David's deer were kept on the same farm.All animals were fed with the same whole mixed diet every day.The pre-trial period lasted for 7 days and the full trial period for 21 days.On the 1st day after trial period finished,sika deer,reindeer and Père David's deer were anaesthetized using xylazine hydrochloride after morning feeding.RNA-seq technique was used to compare the differences in blood gene expression among them.The principal components analysis(PCA)results showed that the blood gene expression patterns were significantly different among sika deer,reindeer and Père David's deer.Compared with reindeer,2 932 differently expressed genes(DEGs)were identified in the blood of sika deer,which were enriched in GO terms such as very long-chain fatty acids metabolic process and KEGG pathways such as peroxisome proliferator-activated receptor(PPAR)signaling pathway.Compared with Père David's deer,6 765 DEGs were identified in the blood of sika deer,which were enriched in GO terms such as lipoprotein metabolic process and KEGG pathways such as ribosome.Compared with Père David's deer,5 930 DEGs were identified in the blood of reindeer,which were enriched in GO terms such as protein secretion and KEGG pathways such as biosynthesis of unsaturated fatty acids.In conclusion,the body gene expression patterns of sika deer,reindeer and Père David's deer were significantly different under the same feeding condition,which may provide a reference for comprehending the gene expression patterns of cervidea.
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