In order to study the isolation and culture method of sika deer fibroblasts and establish a seed bank,this study analyzed and compared the efficiency of isolation and culture of sika deer primary fibroblasts by enzyme digestion culture method and tissue block culture method,and proved that the isolation and culture of sika deer fibroblasts had achieved better results by the enzyme digestion culture method,and a sika deer fibroblast seed bank has been established through primary culture,freezing and recovery.The research aims to lay the foundation for the preservation of sika deer germplasm resources,somatic cell nuclear transplantation and gene cloning research.
维普
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