Effects of Antler Stem Cells on Regeneration and Repair of Sciatic Nerve Injury in Rats
To investigate the repairing effect of antler stem cells on sciatic nerve injury in rats,30 male SD rats were prepared to create sciatic nerve injury model and randomly divided into three groups:antler stem cell(ASC)transplantation group,rat bone marrow mesenchymal stem cell(rBMSC)transplantation group and saline control group,with 10 rats in each group.The stem cell transplantation group was treated by injecting 1 × 106 cells(suspended with normal saline)into the tail vein,while the control group was given the same dose of normal saline.Samples were collected at day 7 and day 21 after treatment.Behavioral observation and footprint analysis were conducted to detect the recovery of nerve function.Histological staining was used to evaluate the pathological changes of sciatic nerve and the recovery of gastranemius atrophy on the affected side.The expression levels of NF200,c-Jun,Periaxin,MPZ and Krox20 related to sciatic nerve regeneration were detected by immunofluorescence staining and RT-qPCR.The results showed at 3 weeks after surgery,sciatic nerve function and nerve regeneration were significantly improved in the stem cell transplantation group compared with those in the control group,and the effect was more significant in the ASC transplantation group.SFI data showed that at 3 weeks after surgery,the SFI value of ASC group was significantly better than that of rBMSC group and control group.At 21 days after surgery,the wet-weight ratio and muscle fiber area of gastrocnemius of the affected side of the control group were significantly lower than those of the stem cell transplantation group,and the rBMSC transplantation group was significantly lower than that of the ASC transplantation group.Histological staining results showed that the number of regenerated nerve fibers in ASC transplantation group increased significantly,the arrangement was regular,and the expression level of NF200 increased significantly.At day 7 after surgery,RT-qPCR results showed that ASC transplantation significantly enhanced c-Jun,Periaxin,Mpz and Krox20 gene expression compared with those in the control group.Based on the biological phenomenon that antler nerve can regenerate periodically,this study is the first time to explore the effect of antler stem cells on nerve regeneration and repair in rats,and confirmed that ASC transplantation significantly promoted the regeneration and repair of sciatic nerve injury and the recovery of motor function in rats,and the effect was even better than that of rBMSC transplantation group.
antlerstem cellsciatic nerve injuryregeneration and repair
万方数据
维普
