Deer Antler Stem Cell-Derived Conditioned Medium Improves Psoriasis-Like Skin Lesions in Mice via Regulating the NF-κB Pathway
The aim of this study was to investigate the effects and underlying mechanism of deer antler stem cell secretions and its conditioned medium(AnSC-CM)on imiquimod-induced psoriasis-like skin lesions in mice.A psoriasis mouse model was created using imiquimod cream.40 mice were then divided into a model group,a positive treatment group using bone marrow mesenchymal stem cell-derived conditioned medium(BMSC-CM),an AnSC-CM treatment group,and a normal control group,10 mice in each group.The severity of skin lesions was observed morphologically,and the psoriasis area and severity index(PASI)of skin lesions were analyzed in each group of mice.Histopathological changes in the damaged skin in each group of mice were examined using HE staining.Immunohistochemical staining was performed to assess the effects of AnSC-CM on abnormal proliferation and inflammation of mouse epidermal cells.Western blotting was used to detect the activation of the nuclear transcription factor-KB(NF-κB)pathway.The results showed that compared with the normal group,the model group mice showed significant psoriasis lesions,and the erythema,scale,skin lesion thickness,total PASI score and epidermal thickness were all extremely significantly increased(P＜0.01).Compared with model group,the symptoms of skin lesions in both AnSC-CM and BMSC-CM groups were reduced,and the erythema,scale,skin lesion thickness,total PASI score and epidermal thickness were all extremely significantly decreased(P＜0.01),and the effect of AnSC-CM was better than that of BMSC-CM.Further research found that AnSC-CM also significantly inhibited abnormal proliferation of epidermal cells,and the expression levels of KRT14,PCNA,indoleamine 2,3-dioxygenase(IDO),and CD163 in psoriasis-like skin lesions of AnSC-CM group were extremely significantly reduced(P＜0.01),and the expression levels of NF-κB signaling pathway related proteins(p65,p-p65,IKB,and p-IKB)were extremely significantly reduced(P＜0.01).The results of this study showed that AnSC-CM may play a crucial role in alleviating psoriasis by inhibiting the activation of the NF-κB pathway,thereby inhibiting the occurrence of inflammation.
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