Pharmacological Mechanism of Deerskin Gum in Promoting Skin Healing
To study the healing effect of deerskin gum of different concentrations on the wound surface of skin defect in rats,to clarify the pharmacological mechanism of deerskin gum in promoting skin healing,and to investigate the biological effects of deerskin gum on pilose antler mesenchymal cells cultured in vitro,the body weight,wound surface morphology,wound healing rate,epidermal growth factor receptor protein(EGFR),heparin-bound epidermal growth factor(HB-EGF),hydroxyproline HYP,histological observation,proliferating cell nuclear antigen(PCNA)and vascular endothelial growth factor(VEGF)contents were observed.Different concentrations of deer skin gelatin were added to the medium of pilose antler mesenchymal cells,and bovine serum albumin(BSA),insulin-like growth factor(IGF-Ⅰ)and different concentrations of AJ were added to observe the proliferation and differentiation of pilose antler mesenchymal cells.The results showed that different concentrations of deerskin gum could promote the wound healing of skin defect in rats,and the effect of the middle and high dose group was the best,which was similar to that of the positive control group.At low dose of 0.5-1 mg/mL and high dose of 20-40 mg/mL,deer gum could inhibit the proliferation and promote apoptosis of pilose antler mesenchymal cells.At the concentration of 5-10 mg/mL,deer gum could stimulate the proliferation of pilose antler mesenchymal cells.These results clarified that the mechanism of deerskin glue promoting wound healing of rat skin defect was to promote angiogenesis,enhance vascular permeability and promote wound healing by regulating epidermal cells and fibroblasts.Deer gum has a bi-directional regulatory effect on deer antler mesenchymal cells cultured in vitro.
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维普
