为探究不同锯别鹿茸的抗炎作用,选取梅花鹿初角茸、头锯二杠茸、二锯二杠茸、三锯二杠茸、四锯二杠茸、五锯二杠茸、六锯三权茸、七锯三杈茸各3副,粉碎后用胃蛋白酶和胰蛋白酶制成的双酶酶解液处理后进行药理试验.动物试验选取周龄及体重相近的雄性昆明小鼠66只,随机分为11组(n=6),即空白对照组、脂多糖(LPS)组、蒲地蓝组、初角茸(A组)、头锯二杠茸(B组)、二锯二杠茸(C组)、三锯二杠茸(D组)、四锯二杠茸(E组)、五锯二杠茸(F组)、六锯三权茸(G组)、七锯三权茸(H组),A～H组均使用质量浓度为260 mg/mL的酶解液进行灌胃(0.3 mL/d),连续14d.除空白对照组以外,所有试验组在第15天均注射0.3 mL LPS溶液,第15天进行采血和解剖取样,检测血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)4个炎症因子的含量,通过肝脏、肾脏石蜡切片观察组织病变情况.血清检测结果显示:F,G组血清IL-1β含量极显著低于LPS组(P＜0.01),F,G,H组血清IL-6含量极其显著低于LPS对照组(P＜0.001);E组血清IL-6含量极显著低于LPS组(P＜0.01),C,D,E,F,G,H组血清IL-8含量极显著低于LPS对照组(P＜0.001),F,G组TNF-α含量极显著低于LPS对照组(P＜0.01).组织切片观察结果显示,F,G组酶解液对LPS诱导的炎症小鼠肝脏组织结构保护作用最好.研究结果表明,各试验组鹿茸酶解液均能降低炎症因子水平,对肝脏和肾脏的炎症反应均有所缓解,起到保护作用,其中五锯二杠茸组的炎症因子水平最低,保护效果最好.试验结果为鹿茸抗炎作用提供参考,可为临床应用和产品开发与评价提供理论依据.
Effects of Different Enzymatic Hydrolysate of Sika Deer Antlers on Inflammatory Mice Induced by LPS
This experiment was aimed to investigate the anti-inflammatory effects of different deer antler harvested with different ages.In this experiment,the initial antlers,two branched antlers from the second to the sixth growing year,three branched antlers from the seventh and eighth growing year were selected,3 pairs in each group respectively.The antlers were crushed and made into a double enzymatic hydrolysate using pepsin and trypsin for subsequent pharmacological tests.Sixty-six male KM mice with similar age and body weight were randomly divided into 11 groups(n=6),i.e.,blank control group,LPS group,pullulan group,the initial antlers(Group A),two branched antlers from the second to the sixth growing year(Group B,Group C,Group D,Group E and Group F),three branched antlers from the seventh(Group G)and eighth(Group H)growing year.A-H groups were all gavaged with the enzymatic hydrolysate 0.3 mL/d at a concentration of 260 mg/mL for 14 consecutive days.All experimental groups,except the blank control group,were injected with lipopolysaccharide(LPS)solution 0.3 mL at 15th day,blood and autopsy samples were taken on the 15th day for the detection of inflammatory factors,including interleukin-1(3(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8)and tumour necrosis factor-α(TNF-α),and tissue observations of paraffin sections of liver and kidney.The results showed that the levels of IL-1β in groups F and G were extremely significantly lower than those in the LPS group(P＜0.01),the levels of IL-6 in groups F,G and H were extremely significantly lower than those in the LPS group(P＜0.001),the levels of IL-6 in group E were highly significantly lower than those in the LPS group(P＜0.01),the levels of serum IL-8 in groups C,D,E,F,G and H were extremely significantly lower than those in the LPS group(P＜0.001),and the levels of TNF-α content in groups F and G were extremely significantly lower than that in the LPS group(P＜0.001).Groups F and G showed the most pronounced effect on the protection of hepatic histological structure of LPS-induced inflammation in the mice.It was finally concluded that all deer antlers enzymatic hydrolysate groups could reduce the levels of inflammatory factors,which had a protective effect on the liver and kidney by alleviating the inflammatory response,among which group F had the lowest levels of inflammatory factors and the best protective effect.The results of this test initially provided a reference for the anti-inflammatory effect of antler velvet,which could provide a certain theoretical basis for clinical application,product development and evaluation.
万方数据
维普
