山东省和吉林省梅花鹿大肠杆菌的分离鉴定和耐药性分析

Investigation of Drug Resistance of Escherichia coli Isolated from Sika Deer in Shandong and Jilin Provinces

张作鹏 ¹蒲允山 ¹程林 ²徐栋 ³秦亚 ⁴刘雪彤 ⁵宫庆龙⁵

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作者信息

1. 青岛市城阳区农业农村局,青岛 266109
2. 青岛市农业农村局,青岛 266071
3. 青岛森林野生动物世界有限公司,青岛 266000
4. 吉林农业大学动物科学技术学院/动物医学院,长春 130118;青岛农业大学动物医学院,青岛 266109
5. 吉林农业大学动物科学技术学院/动物医学院,长春 130118
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摘要

为探究山东省和吉林省梅花鹿源大肠杆菌的耐药性与耐药基因携带情况,从2省梅花鹿的30份粪便中分离鉴定出29株(分离率96.67％)大肠杆菌,测定分离株的最小抑菌浓度(Minimum inhibitory concentration,MIC).结果表明:分离株呈现不同程度的耐药性,对＞3种抗生素耐药的菌株占48.28％,对四环素和氨苄西林的耐药率均＞50％;喹诺酮类qnrS、四环素类tet(A)、β-内酰胺类blaTEM耐药基因的检出率均＞50％,qnrS的检出率达86.21％.吉林省来源分离株的耐药率和耐药基因检出率均高于山东省分离株.

Abstract

The drug resistance and drug-resistant genes of Escherichia coli(E.coli)isolated from sika deer in Shandong and Jilin Province were investigated.A total of 29 E.coli strains(96.67％)were isolated from 30 fecal samples of sika deer in Shandong and Jilin Province,and the minimum inhibitory concentration(MIC)of the isolated strains was determined.The results revealed varying degrees of drug resistance among the isolates,with multi-drug resistant strains accounting for 48.28％to more than three drugs,with a resistance rate exceeding 50％to tetracycline,ampicillin,etc.Moreover,quinolones qnrS,tetracyclines tet(A),and β-lactam blaTEM resistance genes were detected at rates above 50％,with qnrS being detected as high as 86.21％.Notably,both the drug resistance rate and detection rate of drug-resistant genes in Jilin Province surpassed those in Shandong Province.

关键词

梅花鹿/大肠杆菌/耐药表型/耐药基因

Key words

sika deer/Escherichia coli/resistance phenotype/resistance gene

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基金项目

吉林省教育厅科学技术研究项目(JJKH20230410KJ)

出版年

2024

经济动物学报

吉林农业大学

经济动物学报

CSTPCD

影响因子：0.822

ISSN：1007-7448

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