Establishment of ISSR-PCR reaction system, primers screening and their applications to genetic diversity analysis in Eucommia ulmoides
In order for the further study on population genetic diversity in Eucomia ulmoides, inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) system was established through orthogonal test for E.ulmoides.Fourteen ISSR polymorphie primers were selected, and genetic diversity was analyzed.A total of 108 polymorphic loci were amplified, showing percentage of polymorphic loci of 100％, effective number of alleles per locus (ne) of 1.505, expected heterozygosity (He) of 0.308, and Shannon's information index (Ⅰ) of 0.472.The results indicate that E.ulmoides has high level of genetic diversity, and ISSRs are suitable markers for genetic diversity analysis in E.ulmoides.
NETL
NSTL
万方数据
维普
