Variation of endogenous hormone in Cornus walteri seeds during cold stratiifcation and its relationship with germination
In order to analyze the relationship between endogenous hormone and germination of Cornus walteri seeds, to learn mechanism of seed dormancy, variation of endogenous hormone contents in C. walteri seeds during cold stratiifcation were studied with enzyme linked immunosorbent assay (ELSA). The results show that IAA content drops in the early phase of cold stratiifcation, and then is signiifcantly increased after a certain duration, but is decreased at the last stage, and ratio variation of IAA to ABA is so. ABA content is higher in the early phase of cold stratification, and then is decreased with extension of treatment time. Variation of GA1/3 is relative steady, and it gradually rise as a whole but some fluctuations. ZRs content has some small variations and nearly a straight line. iPAs content has relative bigger variations, and show M-shaped curve during whole treating period. The results of correlation analysis indicate that IAA content has very signiifcantly positive relationship with ratio of IAA to ABA (p < 0.01). ABA content has very signiifcantly negative relationship with ratio of ZRS to ABA (p < 0.01), and significant negative relationship with ratio of iPA to ABA (p < 0.05). The results of seed germination test show that germination rate and germination potential of seeds are increased obviously after stratification, and are strengthened with prolonging treating time. Germination rate of seeds without stratification is zero, germination rate and germination potential of seeds treated for 180 days are 41.34%and 36.13%respectively, which are the maximum in all treatments. It need go through above 150 days’ cold stratiifcation to relieve deep dormancy of C.walteri seed.
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NSTL
万方数据
维普
