首页|对节白蜡树叶乙酸乙酯提取物的GC-MS分析及其结构初探

对节白蜡树叶乙酸乙酯提取物的GC-MS分析及其结构初探

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为了研究对节白蜡的抗病原因,以探寻新的生物农药,在前期有关对节白蜡树叶提取物杀菌活性研究的基础上,对其乙酸乙酯提取物及柱层析分离组分进行了GC-MS分析,初步确认了各组分所含化合物的可能结构.结果表明:乙酸乙酯提取物中面积归一含量超过1%的化合物组分有18个,其中3号化合物的面积归一含量为17.13%,其结构为4-羟苯基乙醇;经过柱层析分离后各组分所含化合物要相对集中和更加纯化些,其中组分A所含物质主要有两个,化合物4-羟苯基乙醇的含量达到了81.3%,这是相对比较纯的物质;作者据其已有的相关研究结果(组分A整体表现出的杀菌活性要优于其他组分)分析认为,4-羟苯基乙醇作为对节白蜡树叶中所含的重要化合物,在对节白蜡的抗病方面起到了重要作用.
GC-MS analysis and preliminary study on structures of extracts from Fraxinus hupehensis leaves
In order to study the anti-disease cause of Fraxinus hupehensis in search of a new bio-pesticide,on the basis of the early bactericidal activity researches on extract from F.hupehensis leaves,ethyl acetate extracts and its column chromatography isolation ingredients were analyzed by GC-MS,and the possible structures of compounds contained in each ingredient were preliminarily identified.The results showed that,in ethyl acetate extracts,area normalization contents of 18 compound ingredients were over 1%,among which the No.3 compound ingredient had area normalization contents of 17.13% and had the structure of 4-hydroxyl phenyl ethanol.Through column chromatography isolation,compounds contained in each ingredient were more concentrated and purer.Ingredient A contained two substances,and its 4-hydroxyl phenyl ethanol content reached 81.3%,which was relatively pure.According to available relevant research results,the bactericidal activity shown by Ingredient A was better than that of the others.So 4-hydroxyl phenyl ethanol,as a important compound in F.hupehensis leaves,played an important role in the anti-disease ability ofF.hupehensis.

Fraxinus hupehensisextractfungicide activityGC-MSstructure

张良菊、徐志红、徐慧慧、李俊凯、吴清来

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长江大学农学院,湖北荆州434025

长江大学长江大学农药研究所,湖北荆州434025

对节白蜡 提取物 杀菌活性 气质联用 结构

国家自然科学基金湖北省自然科学基金

309719482014CF105

2017

经济林研究
中南林业科技大学

经济林研究

CSTPCD北大核心
影响因子:1.423
ISSN:1003-8981
年,卷(期):2017.35(3)
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