Identification and analysis of CCCH type zinc finger protein gene family in Camellia oleifera under cold stress
[Objective]To provide reference for the mining and utilization of genetic resources of cold-resistant Camellia oleifera.[Method]Based on the cold stress transcriptome data of C.oleifera,the CCCH-type zinc finger protein gene was identified,and the physicochemical properties,phylogenetic analysis,conserved domain prediction and secondary structure prediction of CCCH-type zinc finger protein were analyzed.The high-altitude C.oleifera(LSG)of Lushan mountain and the cultivated C.oleifera Gan Wu1(GW1)were used as materials.The expression of CCCH-type zinc finger protein gene under cold stress was analyzed.[Result]A total of 24 CCCH-type zinc finger protein genes were identified in the cold stress transcriptome data of common C.oleifera,and the 24 CCCH-type zinc finger proteins were clustered into 2 subfamilies with reference to the rice classification method.Conservative motif analysis showed that there were only three motif types of CCCH-type zinc finger proteins among the 24 genes,viz,C-X8-C-X5-C-X3-H,C-X7-C-X5-C-X3-H,and C-X5-C-X4-C-X3-H,with numbers of 92,4,and 4,respectively.The results of transcriptome expression profiling showed that in the field cold stress test,CoC3H17 gene expression was higher than that of D1,D2 and D3 in the D4,D5 and D6 periods,and the CoC3H17 gene of LSG and GW1 consistently showed higher transcript abundance in the indoor cold stress test;the CoC3H14 and CoC3H24 genes showed a significant increase in expression in the D4,D5 and D6 periods of the field test and in the indoor cold stress test,and the expression of these two genes in LSG was higher than that of GW1.[Conclusion]The CoC3H17,CoC3H14 and CoC3H24 genes may be the key genes in the response of C.oleifera to response to cold stress.
Camellia oleiferaCCCH type zinc finger protein gene familybioinformatics analysiscold stresstranscriptome
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NSTL
万方数据
