Evaluation of cold resistance of 9 kinds of apple shoots combined with scion-rootstock in Aksu area
[Objective]To evaluate the cold resistance of 9 kinds of apple scion-rootstock combination,and to screen out the combination of scion-rootstock with excellent cold resistance,so as to provide the basis for the selection of apple's excellent scion-rootstock combination in Aksu area.[Method]The 1-year deep dormant apple shoots of each anvil-ear combination of TD-1/Orin,Malus robusta/Venus gold,M.robusta/Honey Crisp,M.robusta/Xinhong 1,M.robusta/Envy,M9T337/Envy,Qingzhen/Ruixue,Malus sieversii/Xinhong 2 and M.sieversii/Xinhong 1 in Hongpo Farm were used as test materials.The branches were treated at-15,-20,-25,-30,-35 and-40℃ for 12 h,and the contents of relative electrical conductivity,malondialdehyde(MDA),soluble protein and free proline(PRO)physiological indexes of catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities were measured at 4℃.The cold resistance of 9 kinds of apple branches combined with scion-rootstock was evaluated by the method of conductivity combined with Logistic equation and membership function.[Result]With the decrease of treatment temperature,the relative electrical conductivity of 9 kinds of apple rootstock-scion combination increased in an"S"shape curve,and the relative electrical conductivity of Qingzhen/Ruixue and M.robusta/Venus gold were significantly higher than other combinations.MDA showed a bimodal trend,and the content of MDA was significantly higher than that of other combinations,the peak content of MDA was 4.2 times that of CK.The activities of soluble protein,PRO,and protective enzymes CAT,POD and SOD basically increased first and then decreased,and were negatively correlated with the relative conductivity.The average soluble protein content of M.sieversii/Xinhong 1 and M.sieversii/Xinhong 2 was significantly higher than that of other combinations.The average PRO content of M.sieversii/Xinhong 1 and M.robusta/Honey Crisp was significantly higher than that of other anvil-spike combinations,the CAT content of M.sieversii/Xinhong 2 was significantly higher than that of other combinations,the peak content was 5.9 times of CK,and the total POD activity of M.sieversii/Xinhong 2 and M.robusta/Xinhong 1 was significantly higher than that of other combinations.The average POD activity of M.sieversii/Xinhong 2 and M.robusta/Xinhong 1 combined branches was significantly higher than that of other combinations,and the average SOD activity of M.sieversii/Xinhong 2,M.robusta/Xinhong 1,M.sieversii/Xinhong 1 and M.robusta/Honey Crisp combined branches was significantly higher than that of other combinations.They were 3.25,3.43,2.92 and 3.79 times of those treated at 4℃,respectively.[Conclusion]The cold tolerance of 9 kinds of apple anvil-ear combinations from strong to weak was M.sieversii/Xinhong 2,M.sieversii/Xinhong 1,M.robusta/Honey Crisp,M.robusta/Xinhong 1,M.robusta/Envy,M9T337/Envy,TD-1/Orin,M.robusta/Venus gold,Qingzhen/Ruixue.
scion-rootstock combinationan apple branchcold resistancelow temperature stresssemi-lethal temperaturemembership function method
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