Pollen biological characteristics of different provenances of Prunus sibirica clones
[Objective]To provide reference for screening excellent pollination varieties of Prunus sibirica.[Method]Taking 30 P.sibirica clones from 8 provenances as the research objects,the flowering phenology was investigated,the pollen quantity and pollen morphology were observed,the pollen viability was detected,and the pollen biological characteristics were analyzed by cluster analysis and principal component analysis.[Result]The flowering period of P.sibiricain was concentrated from 9 April to 17 April.According to the flowering period,the clones were divided into 4 groups.The first group included No.5,No.7 and No.1006,the second group contained clones No.592,No.29,the third group contained clones No.14,No.334,No.506,No.518,No.1004,and the fourth group contained No.508,No.516,No.517,No.352 and other 20 clones.The pollen grains of P.sibirica were prolate or super-prolate,with three narrow and shallow germination grooves.The polar view was divided into two shapes:near-circular and trilobal.The mean pollen deformity rate was 6.08% to 27.14% .The pollen morphological characteristics of different seed sources.The coefficients of variation of the pollen morphology indexes ranged from 1.58% to 15.65%,and the coefficients of variation among seed sources ranged from 2.20% to 9.79% .The single flower pollen amount of each clone of P.sibirica ranged from 10 083 grains to 32 250 grains,among which 17 clones,including clone No.508 and No.592,differed significantly.The pollen germination rates ranged from 7.11% to 95.84%,and the pollen tube lengths ranged from 74.08 μm to 529.97 μm.Among which,clone No.30 had the highest germination rate and the longest pollen tube lengths,which were significantly higher than those of some clones;No.328 and No.17 had lower deformity rates than other clones,and the pollen was fuller.The tested clones could be clustered into six groups.Group Ⅵ had the highest number of single flower pollen,the highest germination rate,the longest pollen tube,and the best comprehensive performance.Groups Ⅳ and Ⅴ had more single flower pollen,higher germination rate,longer pollen tube,and better comprehensive performance.The single flower pollen quantity of groupⅢ was less,the germination rate was low,the pollen tube growth rate was slow,and the comprehensive performance was poor.The single flower pollen quantity,germination rate and pollen tube length of groupsⅠand Ⅱ were medium,and the comprehensive performance was general.The cumulative contribution rate of three principal components of pollen germination rate,pollen tube length,ingle flower pollen quantity and deformity rate was 92.697% .[Conclusion]The pollen characteristics of 30 P.sibirica clones differed significantly,and six clones,No.30,No.328,No.56,No.516,No.508,and No.518 showed excellent performance,among which,No.30 asexual line had the highest score of pollen characteristics,which was suitable for breeding as a pollinated variety.
NETL
NSTL
万方数据
