Identification of the ERF gene family in Lonicera macranthoides and its expression profiles during senescence
[Objective]Lonicera macranthoides is a major authentic Chinese herb and an important economic plant in southern China.Its secondary metabolites,such as chlorogenic acid and luteoloside,accumulate during organ senescence and are regulated by transcription factors.Ethylene signaling related ERF gene family plays an important role in plant senescence,stress responses and secondary metabolism.Identification of ERF family members and exploration of their expression patterns can provide a reference for functional studies on the regulation of secondary metabolism by this class of genes.A comprehensive study of the ERF gene family in L.macranthoides has not yet been reported.[Method]The ERF transcription factor family was identified based on transcriptomic data.A phylogenetic tree was constructed to analyze their evolutionary relationships,and the structural motifs of these sequences were analyzed using the MEME Suite tools.QRT-PCR was used to analyze the expression patterns of LmERF genes in different organs during senescence as well as under cold stress and ethylene treatment.[Result]A total of 65 LmERF genes with conserved structural domains of AP2 were identified in L.macranthoides,which were divided into three subgroups.20 LmERFs were observed to be differentially expressed between young and senescent leaves using RNA-Seq.Further qRT-PCR analysis showed that 18,13 and 11 LmERF genes were significantly differentially expressed during senescence in leaves,stems,and flowers,respectively.Of them,LmERF2,LmERF9,LmERF16,LmERF17 and LmERF19 were remarkably differentially expressed during senescence in all tissues.After low temperature treatment,seven LmERFs,including LmERF1,LmERF2,LmERF6,LmERF7,LmERF8,LmERF11 and LmERF12,were strongly up-regulated,while LmERF3,LmERF9,LmERF16 and LmERF19 were outstandingly down-regulated in leaves and stems.Ethylene treatment induced the expression levels of LmERF1,LmERF8 and LmERF12,while suppressed the expression levels of LmERF2,LmERF4,LmERF9,LmERF10,LmERF14,LmERF15,LmERF16,LmERF17,LmERF18 and LmERF19.[Conclusion]Eight LmERFs(LmERF1,LmERF2,LmERF6,LmERF9,LmERF10,LmERF15,LmERF16 and LmERF19)were responded to organ development,cold stress,and ethylene-induced senescence,which provide the data for the subsequent excavation of target LmERFs for efficiently regulating secondary metabolism in L.macranthoides.
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