Relationship between leaf structure and inclusions of Camellia oleifera and anthracnose resistance
[Objective]To provide a reference for the selection and breeding of anthracnose-resistant Camellia oleifera varieties and the evaluation of resistance.[Method]Eight C.oleifera lines(CL4,CL18,CL40,CL53,CL166,CL150,CL76,and CL102)of the Changlin series were used to investigate the incidence of anthracnose in C.oleifera,and the morphological and histological structure of C.oleifera leaves of each line was analyzed by paraffin section using light microscopy,scanning electron microscopy,and high-performance liquid chromatography,and the correlation between each index and the content of resistance inclusions was analyzed.The differences between the morphological structure and the resistance content in the leaves of each line were analyzed by paraffin sections using scanning electron microscopy and high performance liquid chromatography.[Result]CL150 was a resistant line,while CL76 and CL102 were susceptible lines.C.oleifera leaves belonged to the bifacial leave,with 2-3 layers of fence tissue,the lowest layer of fence tissue was loosely arranged,the upper epidermis and the lower epidermis were single layers of closely arranged irregular cells,and the thickness of the upper epidermis was greater than the thickness of the lower epidermis.There were great differences in the leaf tissue structure between varieties with different anthracnose resistance.The leaf length and leaf circumference of the resistant varieties were larger,and they had thicker lower epidermis,thinner spongy tissue,and a smaller proportion of spongy tissue.Correlation analysis showed a significant negative correlation between the incidence rate and the palisade tissue spongy tissue ratio,and the correlation coefficient was-0.937;and a very significant positive correlation with the thickness of sponge tissue and leaf tissue looseness,with correlation coefficients of 0.900 and 0.952,respectively.Incidence was negatively correlated with leaf length,leaf circumference,and epidermal thickness,with correlation coefficients of-0.777,-0.733,and-0.736.Based on the multiple regression analysis of 6 resistance indexes,an evaluation model with the incidence of anthracnose of C.oleifera as the dependent variable(y)was established:y=584.556 5-0.916 8x2+0.206 2x4-2.734 7x5-5.646 3x6.The model test results had a good fit(P＜0.001,R2=0.997).[Conclusion]The thickness of spongy tissue,the palisade tissue spongy tissue ratio and tissue looseness could be used as the main reference factors for evaluating leaf anthracnose resistance of C.oleifera,and leaf length,leaf circumference and lower epidermis thickness could be used as auxiliary indexes for identification of leaf anthracnose resistance of C.oleifera.Based on the above resistance indexes,an evaluation model of the resistance of C.oleifera to anthracnose was established with leaf circumference,spongy tissue thickness,palisade tissue spongy tissue ratio and tissue looseness as variables.
NETL
NSTL
万方数据
