Proteome differences between Camellia drupifera and Camellia meiocarpa seed kernels
[Objective]Exploring key differential proteins in Camellia drupifera and Camellia meiocarpa regulating oil synthesis,accumulation and metabolism in seed kernels.[Method]The proteomics of the two seed kernels were identified using label-free technology,the screening and functional classification of differentially expressed proteins were performed by bioinformatics techniques such as subcellular localisation analysis,structural domain analysis,volcano diagram,clustering heatmap,GO and KEGG analysis,and protein-protein interaction networks.[Result]A total of 5 712 proteins were identified in two kinds of seed kernels,and the expression of 740 proteins was significantly different,of which 337 were up-regulated and 403 were down-regulated in C.drupifera seed kernels.GO functional classification results indicated that the differentially expressed proteins were mainly involved in oxidation-reduction process as well as the composition of ribosomes.KEGG enrichment analysis revealed that retinol metabolism,pyruvate metabolism,naphthalene degradation,oxidative phosphorylation,and ribosome were the key metabolic pathways significantly enriched in differentially expressed proteins.Six significantly differentially expressed proteins,starch synthase,glucose-1-phosphate adenylyltransferase,6-phosphotrihalose trihalose synthase/phosphatase,UTP-glucose-1-phosphate uridylyltransferase,fructokinase and hexokinase were down-regulated in starch and sucrose metabolic pathway in the seed kernels of C.drupifera compared to C.meiocarpa,which may be related to fatty acid synthesis and accumulation.In addition,11 significantly differentially expressed proteins were found involved in 4 important pathways related to fatty acid metabolism in the seed kernels of C.drupifera,3 of which were involved in more than one pathway,long-chain acyl-CoA synthetase and mitochondrial enoyl-[acyl-carrier protein]reductase/trans-2-enoyl-CoA reductase were down-regulated in fatty acid biosynthesis,fatty acid degradation and fatty acid elongation pathways,while acyl-[acyl-carrier-protein]desaturase is down-regulated in the fatty acid biosynthesis pathway and up-regulated in the unsaturated fatty acid biosynthesis pathway.[Conclusion]Proteins associated with sugar and fatty acid metabolism were significantly differentially expressed in the seed kernels of C.drupifera,reveales the differences in the accumulation and metabolic patterns of sugar and oil in the seed kernels of C.drupifera and C.meiocarpa,speculate that the decrease in the expression of sugar synthesis related proteins was associated with the conversion of sugars into fatty acids in the seed kernels of C.oleifera.Differential proteins were significantly enriched in the unsaturated fatty acid biosynthesis and retinol metabolism pathways,which may be the biological basis for the unsaturated fatty acid and vitamin enrichment of Camellia oil.
万方数据
维普
