Effects of waterlogging stress and restoration on morphological and physiological characteristics of Xanthoceras sorbifolia Bunge seedlings
[Objective]We investigated the effects of different days of flooding and recovery on the morphological and physiological characteristics of the plants,and explored their adaptability under flooding stress,with a view to providing reference for improving the survival rate of Xanthoceras sorbifolia Bunge plantations and water management during the seedling period in the southern region.[Method]The X.sorbifolia Bunge seedlings were used as the material.The double set of potting method was used to simulate the flooded environment.A total of 5 treatments were set up,namely,0 days of flooding(CK),1 day of flooding(W1),3 days of flooding(W3),5 days of flooding(W5),and 7 days of flooding(W7),and the five treatment groups returned to normal water management for 7 days after the flooding.The changes of seedling leaves in different treatment groups were observed.The changes of physiological indexes such as root activity,leaf osmotic regulator content,antioxidant enzyme activity and photosynthetic pigment content were compared among different treatment groups.[Result]With the extension of the flooding treatment time,the leaves of the seedlings gradually turned yellow and shriveled,and even showed waterlogging symptoms such as wilted and withered,the root activity and leaf photosynthetic pigment content of the seedlings gradually decreased,in which,compared with the control,the root vigour of the W1,W3,W5 and W7 treatment groups were reduced by 22.8%,37.5%,50.5%,and 79.0%,and the total chlorophyll content was reduced by 2.8%,21.5%,27.1% and 33.5%,and carotenoid content decreased by 1.8%,16.6%,22.1% and 34.5%,respectively;the contents of malondialdehyde and soluble sugar in the leaves gradually increased,in which,malondialdehyde content increased by 31.3%,73.0%,84.4% and 112.8% in the W1,W3,W5 and W7 treatment groups,respectively,compared with the control,and soluble sugar content increased by 59.6%,76.4%,106.4% and 127.1%,respectively;while the soluble protein content,proline content,superoxide dismutase activity and peroxidase activity all showed a trend of first increasing and then decreasing,and all peaked in the W3 treatment group,which,compared with the control,increased by 41.4%,76.1%,83.4% and 55.6%,respectively.After 7 days of normal water management,all the indexes of W1 treatment seedlings recovered to the control level,while the indexes of the remaining 3 treatment groups were significantly different from the control.[Conclusion]In summary,after short-term flooding(W1)and normal water management for 7 days,all physiological indexes of seedlings could return to normal levels,while after long-term flooding(W3,W5,W7),all indicators of plants could not return to normal levels.
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